Randomized cross-sectional study to compare HIV-1 specific antibody and cytokine concentrations in female genital secretions obtained by menstrual cup and cervicovaginal lavage
| Autor: | Sahil. Tulsi, Lenine J. P. Liebenberg, Natasha Samsunder, Sarah. Dlamini, Nivashnee Naicker, Nigel Garrett, Nelisile. Majola, Lise. Werner, Lynn Morris, Thomas J. Hope, Jo-Ann S. Passmore, Salim S. Abdool Karim, Derseree. Archary |
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| Přispěvatelé: | Institute of Infectious Disease and Molecular Medicine, Faculty of Health Sciences |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
HIV antibodies--Testing
Epidemiology HIV Antigens medicine.medical_treatment Female contraception lcsh:Medicine HIV Infections Cervix Uteri HIV Antibodies Diagnostic specimens--Collection and preservation Immunoglobulin G law.invention Cohort Studies Nerve growth factor law lcsh:Science Public health Multidisciplinary biology Mucous membrane Body Fluids Cytokine medicine.anatomical_structure Specimen collection Vagina Cytokines Female Antibody Research Article Adult HIV prevention Antibodies Specimen Handling medicine Humans Immunity Mucosal Secretion Mucous Membrane business.industry lcsh:R HIV Cross-Sectional Studies Menstrual cup HIV infections—Prevention Immunology biology.protein HIV-1 lcsh:Q business |
| Zdroj: | PLoS One PLoS ONE PLoS ONE, Vol 10, Iss 7, p e0131906 (2015) |
| DOI: | 10.1371/journal.pone.0131906 |
| Popis: | Introduction Optimizing methods for genital specimen collection to accurately characterize mucosal immune responses is a priority for the HIV prevention field. The menstrual cup (MC) has been proposed as an alternative to other methods including cervicovaginal lavage (CVL), but no study has yet formally compared these two methods. Methods Forty HIV-infected, antiretroviral therapy-naive women from the CAPRISA 002 acute HIV infection cohort study were randomized to have genital fluid collected using the MC with subsequent CVL, or by CVL alone. Qualitative data, which assessed levels of comfort and acceptability of MC using a 5-point Likert scale, was collected. Luminex multiplex assays were used to measure HIV-specific IgG against multiple gene products and 48 cytokines. Results The majority (94%) of participants indicated that insertion, wearing and removal of the MC was comfortable. Nineteen MCs with 18 matching, subsequent CVLs and 20 randomized CVLs were available for analysis. Mucosal IgG responses against four HIV-antigens were detected in 99% of MCs compared to only 80% of randomized CVLs (p = 0.029). Higher specific antibody activity and total antibodies were observed in MCs compared to CVL (all p |
| Databáze: | OpenAIRE |
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