Direct modulation of volume-regulated anion channels by Ca2+chelating agents
| Autor: | Loic Lemonnier, Natacha Prevarskaya, Yulia V. Vitko, Roman Skryma, Yaroslav M. Shuba, F. Vanden Abeele |
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| Rok vydání: | 2002 |
| Předmět: |
Stereochemistry
Biophysics Biochemistry Ion chemistry.chemical_compound Swelling-activated chloride current BAPTA Chloride Channels Structural Biology Negative charge LNCaP Tumor Cells Cultured Genetics Humans Chelation Egtazic Acid Molecular Biology IC50 Edetic Acid Chelating Agents Volume-regulated anion channel Chemistry EDTA Cell Biology Hydrogen-Ion Concentration Calcium chelating agent Blockade LNCaP prostate cancer cell Calcium |
| Zdroj: | ResearcherID |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/s0014-5793(02)02863-6 |
| Popis: | Ca 2+ chelating agents are widely used in biological research for Ca 2+ buffering. Here we report that BAPTA, EDTA and HEDTA produce fast, reversible, voltage-dependent inhibition of swelling-activated Cl 3 current (ICl;swell) in LNCaP prostate cancer epithelial cells that is unrelated to their Ca 2+ binding. BAPTA was the most effective (maximal blockade 67%, IC50 =7 0 W WM, at +100 mV) followed by EDTA and HEDTA. ICl;swell blockade by EDTA was pH-dependent. BAPTA blocked ICl;swell also in other cell types. We conclude that Ca 2+ chelating agents block ICl;swell by acting directly on the underlying channel, and that the negative charge of the free chelator form is critical for the blockade. 4 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies. |
| Databáze: | OpenAIRE |
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