The MYB Activator WHITE PETAL1 Associates with MtTT8 and MtWD40-1 to Regulate Carotenoid-Derived Flower Pigmentation in Medicago truncatula
| Autor: | Lifang Niu, Hao Lin, Wenkai Ji, Chengcai Chu, Yingying Meng, Yiqin Wang, Chongnan Wang, Butuo Zhu, Jiangqi Wen, Million Tadege, Huan Liu, Zuoyi Wang |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Lutein Flowers Plant Science Genes Plant 01 natural sciences In Brief Anthocyanins 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Medicago truncatula Basic Helix-Loop-Helix Transcription Factors MYB Gene Carotenoid Research Articles Plant Proteins chemistry.chemical_classification Regulation of gene expression Base Sequence biology Arabidopsis Proteins Pigmentation Activator (genetics) organic chemicals fungi food and beverages Cell Biology Plants Genetically Modified biology.organism_classification Carotenoids Biosynthetic Pathways Cell biology Phenotype 030104 developmental biology chemistry Petal Transcription Factors 010606 plant biology & botany |
| Zdroj: | The Plant Cell. 31:2751-2767 |
| ISSN: | 1532-298X |
| Popis: | Carotenoids are a group of natural tetraterpenoid pigments with indispensable roles in the plant life cycle and the human diet. Although the carotenoid biosynthetic pathway has been well characterized, the regulatory mechanisms that control carotenoid metabolism, especially in floral organs, remain poorly understood. In this study, we identified an anthocyanin-related R2R3-MYB protein, WHITE PETAL1 (WP1), that plays a critical role in regulating floral carotenoid pigmentation in Medicago truncatula. Carotenoid analyses showed that the yellow petals of the wild-type M. truncatula contained high concentrations of carotenoids that largely consisted of esterified lutein and that disruption of WP1 function via Tnt1 insertion led to substantially reduced lutein accumulation. WP1 mainly functions as a transcriptional activator and directly regulates the expression of carotenoid biosynthetic genes including MtLYCe and MtLYCb through its C-terminal acidic activation motif. Further molecular and genetic analyses revealed that WP1 physically interacts with MtTT8 and MtWD40-1 proteins and that this interaction facilitates WP1’s function in the transcriptional activation of both carotenoid and anthocyanin biosynthetic genes. Our findings demonstrate the molecular mechanism of WP1-mediated regulation of floral carotenoid pigmentation and suggest that the conserved MYB-basic-helix-loop-helix-WD40 regulatory module functions in carotenoid biosynthesis in M. truncatula, with specificity imposed by the MYB partner. |
| Databáze: | OpenAIRE |
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