Analysis of the role of the human papillomavirus 16/18 E7 protein assay in screening for cervical intraepithelial neoplasia: a case control study
| Autor: | Lan Zhu, Xiaoping Xiao, Shuhui Song, Huiping Lou, Caijuan Li, Linghua Kong, Ying Zhang, Honghui Shi, Xin Yu, Yan Li, Tao Xu, Moudu Liu, Ruoli Guan, Qingbo Fan, Pengfei Liu, Haiyuan Liu |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Oncology Cancer Research medicine.medical_specialty genetic structures Concordance E7 oncoprotein Cervical intraepithelial neoplasia lcsh:RC254-282 03 medical and health sciences 0302 clinical medicine Surgical oncology Internal medicine Genetics medicine Humans Pathological Early Detection of Cancer Cervical cancer Human papillomavirus 16 medicine.diagnostic_test Human papillomavirus 18 business.industry Case-control study Correction virus diseases Gold standard (test) medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Uterine Cervical Dysplasia Cervical intraepithelial neoplasia (CIN) High-risk human papillomavirus (Hr-HPV) female genital diseases and pregnancy complications 030104 developmental biology nervous system 030220 oncology & carcinogenesis Immunoassay Case-Control Studies Female business psychological phenomena and processes Research Article |
| Zdroj: | BMC Cancer BMC Cancer, Vol 20, Iss 1, Pp 1-7 (2020) |
| DOI: | 10.21203/rs.3.rs-39820/v2 |
| Popis: | Background Cervical cancer is the second-most common gynecological cancer, early screening plays a key role in the diagnosis and treatment of cervical intraepithelial neoplasia (CIN). Sustained E7 protein expression is the pathological basis for CIN and cervical cancer. Methods We collected the cervical cell samples of women who visited the gynecological clinic of Peking Union Medical College Hospital between September 2018 and September 2019 and submitted them to the high-risk human papillomavirus (Hr-HPV) test. We performed a magnetic particle–based chemiluminescence enzyme immunoassay to analyze the HPV16/18 E7 protein level in CIN of different severities and compared the results with those of cervical pathology (gold standard) and the HPV test. Results The positive rate of HPV16/18 E7 protein increased with the severity of CIN: 26.6% in normal tissue, 58.3% in CIN1, and 70.6% in CIN2 or higher (CIN2+). For CIN2+, the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of the E7 protein were 70.6, 67.9, 52.2, and 82.3%, respectively. These values of the HPV test were 86.8, 44.5, 43.7, and 87.1%, respectively. With the combination of the E7 protein assay and HPV test, the specificity for diagnosing CIN2+ was 78.1%, which was significantly higher than that of the HPV test alone. Conclusions HPV16/18 E7 protein level is correlated with the severity of CIN and has a high concordance rate with the pathological result. For cervical cancer screening, the combination of HPV16/18 E7 protein assay and HPV test improves the CIN diagnostic specificity, detection rate, and detection accuracy. |
| Databáze: | OpenAIRE |
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