Ornithine Decarboxylation System of Shewanella baltica Regulates Putrescine Production and Acid Resistance
| Autor: | Yan Zhang, Yanbo Wang, Wang Feifei, Xingyue Bao, Linglin Fu, Yang Rendi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Ornithine
0303 health sciences Shewanella biology 030306 microbiology Decarboxylation Transporter biology.organism_classification Microbiology Ornithine decarboxylase 03 medical and health sciences chemistry.chemical_compound chemistry Biochemistry Extracellular Putrescine Secretion 030304 developmental biology Food Science Shewanella baltica |
| Zdroj: | Journal of food protection. 84(2) |
| ISSN: | 1944-9097 |
| Popis: | Shewanella baltica, one of the dominant spoilers of seafoods, can synthesize putrescine from ornithine under acidic conditions, which could result in food spoilage and health problems. We identified three regulatory enzymes (SpeC, SpeF, and PotE) in the ornithine decarboxylation (ODC) pathway of S. baltica by searching the NCBI database and exploring their functional roles through gene knock-out technology. The ornithine decarboxylase SpeC is an auxiliary adjustor of the ODC system, whereas the ornithine-putrescine transporter SpeE and ornithine decarboxylase SpeF participate in the production of extracellular putrescine. Exogenous addition of ornithine and putrescine promotes the extracellular secretion of putrescine by upregulating the expression of speF and potE. The putrescine biosynthesis and alkalization of cytoplasm is enhanced at weak acidic pH compared with neutral pH, especially at pH 6.0. The maximum upregulation of ODC genes and the optimum decarboxylation activity of SpeF are achieved in a weak acidic environment (pH 6.0), suggesting that the ODC pathway plays an important role in putrescine production and the cytoplasmic acid counteraction of S. baltica. This study contributes to a wider understanding of spoilage mechanisms in food systems and provides theoretical support for developing novel seafood preservation methods. HIGHLIGHTS |
| Databáze: | OpenAIRE |
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