ATP-Responsive Aptamer-Based Metal–Organic Framework Nanoparticles (NMOFs) for the Controlled Release of Loads and Drugs
| Autor: | Yang Sung Sohn, Anna Kozell, Rachel Nechushtai, Wei Ching Liao, Itamar Willner, Xu Yu, Alessandro Cecconello, Wei-Hai Chen |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Materials science
Aptamer Cell 02 engineering and technology 010402 general chemistry 01 natural sciences doxorubicin Biomaterials cytotoxicity drug release nucleic acids targeting Electrochemistry medicine Doxorubicin Cytotoxicity 021001 nanoscience & nanotechnology Condensed Matter Physics Controlled release 0104 chemical sciences Electronic Optical and Magnetic Materials medicine.anatomical_structure Biochemistry Cancer cell Biophysics Nucleic acid 0210 nano-technology Nucleolin medicine.drug |
| Popis: | Nanoparticles consisting of metal–organic frameworks (NMOFs) modified with nucleic acid binding strands are synthesized. The NMOFs are loaded with a fluorescent agent or with the anticancer drug doxorubicin, and the loaded NMOFs are capped by hybridization with a complementary nucleic acid that includes the ATP-aptamer or the ATP-AS1411 hybrid aptamer in caged configurations. The NMOFs are unlocked in the presence of ATP via the formation of ATP-aptamer complexes, resulting in the release of the loads. As ATP is overexpressed in cancer cells, and since the AS1411 aptamer recognizes the nucleolin receptor sites on the cancer cell membrane, the doxorubicin-loaded NMOFs provide functional carriers for targeting and treatment of cancer cells. Preliminary cell experiments reveal impressive selective permeation of the NMOFs into MDA-MB-231 breast cancer cells as compared to MCF-10A normal epithelial breast cells. High cytotoxic efficacy and targeted drug release are observed with the ATP-AS1411-functionalized doxorubicin-loaded NMOFs. |
| Databáze: | OpenAIRE |
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