Use of Oligonucleotides Carrying Photolabile Groups for the Control of the Deposition of Nanoparticles in Surfaces and Nanoparticle Association
Autor: | Ramon Eritja, Brendan Manning |
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Přispěvatelé: | Consejo Superior de Investigaciones Científicas (España), Ministerio de Educación (España), Generalitat de Catalunya, Instituto de Salud Carlos III, European Commission |
Jazyk: | angličtina |
Rok vydání: | 2011 |
Předmět: |
Ultraviolet Rays
Immobilized Nucleic Acids Oligonucleotides Nanoparticle Metal Nanoparticles Nanotechnology 02 engineering and technology Conjugated system 010402 general chemistry Microscopy Atomic Force 01 natural sciences Catalysis Article Nanomaterials Inorganic Chemistry lcsh:Chemistry Gold nanoparticles Sulfhydryl Compounds Physical and Theoretical Chemistry Particle Size Molecular Biology lcsh:QH301-705.5 Spectroscopy chemistry.chemical_classification Photolysis Oligonucleotide Organic Chemistry Photodissociation Temperature General Medicine DNA 021001 nanoscience & nanotechnology Combinatorial chemistry 3. Good health 0104 chemical sciences Computer Science Applications chemistry photolysis lcsh:Biology (General) lcsh:QD1-999 Colloidal gold gold nanoparticles Thiol Gold 0210 nano-technology Conjugate |
Zdroj: | International Journal of Molecular Sciences, Vol 12, Iss 10, Pp 7238-7249 (2011) International Journal of Molecular Sciences Volume 12 Issue 10 Pages 7238-7249 Digital.CSIC. Repositorio Institucional del CSIC instname |
ISSN: | 1422-0067 |
Popis: | An oligodeoxynucleotide hairpin containing a photolabile 2-nitrobenzyl group in the loop and terminated with a thiol function was prepared. The photocleavage of such a hairpin on gold yields a surface activated with a single stranded oligonucleotide which can be utilised to direct the assembly of nanoparticles conjugated with a complementary strand. Analysis of photocleaved surfaces gives nanoparticle coverage one order of magnitude higher than nonphotocleaved surfaces. This illustrates the ability of photocleavable hairpins to direct the assembly of nanomaterials on conducting materials. The conjugation of the photocleavable hairpin to a gold nanoparticle allows the observation of intermolecular interactions between hairpins linked in different nanoparticles, by comparing the thermal dissociations of a hairpin-nanoparticle conjugates at 260 nm and 520 nm. We have also shown that it is possible to permanently alter the physiochemical properties of DNA-nanoparticles by the introduction of a photocleavable group. Indeed for the first time it has been shown that by exposure to UV light the disassembly of nanoparticle aggregates can be induced. This study was supported by the Spanish Ministry of Education (grants BFU2007-63287 and CTQ2010-20541), Generalitat de Catalunya (2009/SGR/208), the Instituto de Salud Carlos III(CIBER-BNN, CB06_01_0019) and the European Community (NANO-3D NMP4-CT2005-014006, FUNMOL, FP7-NMP-213382-2). We acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI). |
Databáze: | OpenAIRE |
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