Use of Oligonucleotides Carrying Photolabile Groups for the Control of the Deposition of Nanoparticles in Surfaces and Nanoparticle Association

Autor: Ramon Eritja, Brendan Manning
Přispěvatelé: Consejo Superior de Investigaciones Científicas (España), Ministerio de Educación (España), Generalitat de Catalunya, Instituto de Salud Carlos III, European Commission
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Ultraviolet Rays
Immobilized Nucleic Acids
Oligonucleotides
Nanoparticle
Metal Nanoparticles
Nanotechnology
02 engineering and technology
Conjugated system
010402 general chemistry
Microscopy
Atomic Force
01 natural sciences
Catalysis
Article
Nanomaterials
Inorganic Chemistry
lcsh:Chemistry
Gold nanoparticles
Sulfhydryl Compounds
Physical and Theoretical Chemistry
Particle Size
Molecular Biology
lcsh:QH301-705.5
Spectroscopy
chemistry.chemical_classification
Photolysis
Oligonucleotide
Organic Chemistry
Photodissociation
Temperature
General Medicine
DNA
021001 nanoscience & nanotechnology
Combinatorial chemistry
3. Good health
0104 chemical sciences
Computer Science Applications
chemistry
photolysis
lcsh:Biology (General)
lcsh:QD1-999
Colloidal gold
gold nanoparticles
Thiol
Gold
0210 nano-technology
Conjugate
Zdroj: International Journal of Molecular Sciences, Vol 12, Iss 10, Pp 7238-7249 (2011)
International Journal of Molecular Sciences
Volume 12
Issue 10
Pages 7238-7249
Digital.CSIC. Repositorio Institucional del CSIC
instname
ISSN: 1422-0067
Popis: An oligodeoxynucleotide hairpin containing a photolabile 2-nitrobenzyl group in the loop and terminated with a thiol function was prepared. The photocleavage of such a hairpin on gold yields a surface activated with a single stranded oligonucleotide which can be utilised to direct the assembly of nanoparticles conjugated with a complementary strand. Analysis of photocleaved surfaces gives nanoparticle coverage one order of magnitude higher than nonphotocleaved surfaces. This illustrates the ability of photocleavable hairpins to direct the assembly of nanomaterials on conducting materials. The conjugation of the photocleavable hairpin to a gold nanoparticle allows the observation of intermolecular interactions between hairpins linked in different nanoparticles, by comparing the thermal dissociations of a hairpin-nanoparticle conjugates at 260 nm and 520 nm. We have also shown that it is possible to permanently alter the physiochemical properties of DNA-nanoparticles by the introduction of a photocleavable group. Indeed for the first time it has been shown that by exposure to UV light the disassembly of nanoparticle aggregates can be induced.
This study was supported by the Spanish Ministry of Education (grants BFU2007-63287 and CTQ2010-20541), Generalitat de Catalunya (2009/SGR/208), the Instituto de Salud Carlos III(CIBER-BNN, CB06_01_0019) and the European Community (NANO-3D NMP4-CT2005-014006, FUNMOL, FP7-NMP-213382-2).
We acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI).
Databáze: OpenAIRE
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