CD36 Protein Is Involved in Store-operated Calcium Flux, Phospholipase A2 Activation, and Production of Prostaglandin E2
| Autor: | Xiong Su, Nada A. Abumrad, Ondrej Kuda, Richard W. Gross, James R. Skinner, Sung Ho Moon, Christopher M. Jenkins |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
CD36 Antigens
medicine.medical_specialty Thapsigargin CD36 chemistry.chemical_element Uridine Triphosphate CHO Cells Biology Calcium Endoplasmic Reticulum Biochemistry Dinoprostone Proinflammatory cytokine Mice chemistry.chemical_compound Cricetulus Cricetinae Internal medicine Calcium flux medicine Animals Humans Enzyme Inhibitors Molecular Biology Inflammation Mice Knockout Mitogen-Activated Protein Kinase 1 Calcium metabolism Mitogen-Activated Protein Kinase 3 Group IV Phospholipases A2 Endoplasmic reticulum Cell Biology Enzyme Activation Metabolism Endocrinology chemistry Cyclooxygenase 2 Unfolded protein response biology.protein Female |
| Zdroj: | Journal of Biological Chemistry. 286:17785-17795 |
| ISSN: | 0021-9258 |
| Popis: | The scavenger receptor FAT/CD36 contributes to the inflammation associated with diabetes, atherosclerosis, thrombosis, and Alzheimer disease. Underlying mechanisms include CD36 promotion of oxidative stress and its signaling to stress kinases. Here we document an additional mechanism for the role of CD36 in inflammation. CD36 regulates membrane calcium influx in response to endoplasmic reticulum (ER) stress, release of arachidonic acid (AA) from cellular membranes by cytoplasmic phospholipase A(2)α (cPLA(2)α) and contributes to the generation of proinflammatory eicosanoids. CHO cells stably expressing human CD36 released severalfold more AA and prostaglandin E(2) (PGE(2)), a major product of AA metabolism by cyclooxygenases, in response to thapsigargin-induced ER stress as compared with control cells. Calcium influx after ER calcium release resulted in phosphorylation of cPLA(2) and its translocation to membranes in a CD36-dependent manner. Peritoneal macrophages from CD36(-/-) mice exhibited diminished calcium transients and reduced AA release after thapsigargin or UTP treatment with decreased ERK1/2 and cPLA(2) phosphorylation. However, PGE(2) production was unexpectedly enhanced in CD36(-/-) macrophages, which probably resulted from a large induction of cyclooxygenase 2 mRNA and protein. The data demonstrate participation of CD36 in membrane calcium influx in response to ER stress or purinergic receptor stimulation resulting in AA liberation for PGE(2) formation. Collectively, these results identify a mechanism contributing to the pleiotropic proinflammatory effects of CD36 and suggest that its targeted inhibition may reduce the acute inflammatory response. |
| Databáze: | OpenAIRE |
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