Polymeric Nanoparticles Containing Taxanes Enhance Chemoradiotherapeutic Efficacy in Non-small Cell Lung Cancer
| Autor: | Seong-Yun Jeong, Heon Joo Park, Eun Kyung Choi, Si Yeol Song, Hye Kyung Chung, Hye-Won Kang, Sa-Won Lee, Joohee Jung, Sungjin Park, Min Hyo Seo |
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| Rok vydání: | 2012 |
| Předmět: |
Male
inorganic chemicals Radiation-Sensitizing Agents Cancer Research Pathology medicine.medical_specialty Lung Neoplasms Paclitaxel Polymers medicine.medical_treatment Mice Nude Docetaxel Nanoconjugates Flow cytometry Ionizing radiation Mice Random Allocation Carcinoma Non-Small-Cell Lung Cell Line Tumor medicine Animals Humans heterocyclic compounds Radiology Nuclear Medicine and imaging Lung cancer Clonogenic assay A549 cell Mice Inbred BALB C Radiation medicine.diagnostic_test business.industry Drug Synergism Chemoradiotherapy medicine.disease Antineoplastic Agents Phytogenic Xenograft Model Antitumor Assays Endocytosis Radiation therapy enzymes and coenzymes (carbohydrates) Oncology Cell culture Microscopy Electron Scanning Cancer research Feasibility Studies Taxoids business medicine.drug |
| Zdroj: | International Journal of Radiation Oncology*Biology*Physics. 84:e77-e83 |
| ISSN: | 0360-3016 |
| DOI: | 10.1016/j.ijrobp.2012.02.030 |
| Popis: | Purpose To reduce the side effects and improve the efficacy of chemoradiation therapy, taxanes were incorporated into polymeric nanoparticles (PNP), and their synergic effect on radiation therapy in non-small cell lung cancer was evaluated. Methods and Materials The properties of PNP-taxanes were characterized by transmission electron microscopy and dynamic light scattering. The chemoradiotherapeutic efficacy of PNP-taxanes was determined by clonogenic assay, cellular morphology, and flow cytometry in A549 cells. In mice bearing A549-derived tumors, the tumor growth delay was examined after the treatment of PNP-taxanes and/or ionizing radiation (IR). Results The PNP-taxanes were found to be approximately 45 nm in average diameter and to have high solubility in water. They showed the properties of active internalization into cells and preserved the anticancer effect of free taxanes. The survival fraction of A549 cells by clonogenic assay was significantly reduced in the group receiving combined treatment of PNP-taxanes and IR. In addition, in vivo radiotherapeutic efficacy was markedly enhanced by the intravenous injection of PNP-taxanes into the xenograft mice. Conclusions We have demonstrated the feasibility of PNP-taxanes to enhance the efficacy of chemoradiation therapy. These results suggest PNP-taxanes can hold an invaluable and promising position in treating human cancers as a novel and effective chemoradiation therapy agent. |
| Databáze: | OpenAIRE |
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