Characterization of the periplasmic redox network that sustains the versatile anaerobic metabolism of Shewanella oneidensis MR-1
Autor: | Alexandra S. Alves, Bruno M. Fonseca, Isabel Pacheco, S. E. Neto, Mónica N. Alves, Afonso Carrêlo, Ricardo Saraiva Louro, Catarina M. Paquete, Cláudio M. Soares |
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Jazyk: | angličtina |
Rok vydání: | 2015 |
Předmět: |
Microbiology (medical)
Cytochrome lcsh:QR1-502 Biology dissociation constant paramagnetic NMR Redox Microbiology lcsh:Microbiology 03 medical and health sciences Electron transfer periplasmic cytochromes Shewanella oneidensis MR-1 Shewanella oneidensis Cytochrome c nitrite reductase Original Research 030304 developmental biology chemistry.chemical_classification 0303 health sciences 030306 microbiology Periplasmic space Electron acceptor biology.organism_classification electron transfer Electron transport chain electrostatics chemistry Biochemistry biology.protein extracellular respiration |
Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, Vol 6 (2015) |
ISSN: | 1664-302X |
DOI: | 10.3389/fmicb.2015.00665 |
Popis: | The versatile anaerobic metabolism of the Gram-negative bacterium Shewanella oneidensis MR-1 (SOMR-1) relies on a multitude of redox proteins found in its periplasm. Most are multiheme cytochromes that carry electrons to terminal reductases of insoluble electron acceptors located at the cell surface, or bona fide terminal reductases of soluble electron acceptors. In this study, the interaction network of several multiheme cytochromes was explored by a combination of NMR spectroscopy, activity assays followed by UV-visible spectroscopy and comparison of surface electrostatic potentials. From these data the small tetraheme cytochrome (STC) emerges as the main periplasmic redox shuttle in SOMR-1. It accepts electrons from CymA and distributes them to a number of terminal oxidoreductases involved in the respiration of various compounds. STC is also involved in the electron transfer pathway to reduce nitrite by interaction with the octaheme tetrathionate reductase (OTR), but not with cytochrome c nitrite reductase (ccNiR). In the main pathway leading the metal respiration STC pairs with flavocytochrome c (FccA), the other major periplasmic cytochrome, which provides redundancy in this important pathway. The data reveals that the two proteins compete for the binding site at the surface of MtrA, the decaheme cytochrome inserted on the periplasmic side of the MtrCAB-OmcA outer-membrane complex. However, this is not observed for the MtrA homologues. Indeed, neither STC nor FccA interact with MtrD, the best replacement for MtrA, and only STC is able to interact with the decaheme cytochrome DmsE of the outer-membrane complex DmsEFABGH. Overall, these results shown that STC plays a central role in the anaerobic respiratory metabolism of SOMR-1. Nonetheless, the trans-periplasmic electron transfer chain is functionally resilient as a consequence of redundancies that arise from the presence of alternative pathways that bypass/compete with STC. |
Databáze: | OpenAIRE |
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