Outer membrane protein A (OmpA) is a potential virulence factor of Vibrio alginolyticus strains isolated from diseased fish
| Autor: | Nattarika Chaichana, Nongyao Sawangjaroen, Patrick C. Y. Woo, Jade L. L. Teng, Hwei Huih Lee, Natthawan Sermwittayawong, Supansa Bunpa, Decha Sermwittayawong |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Virulence Factors Veterinary (miscellaneous) Mutant Virulence Aquatic Science Virulence factor Microbiology 03 medical and health sciences Fish Diseases Animals Electrophoresis Gel Two-Dimensional Vibrio alginolyticus Two-dimensional gel electrophoresis biology Biofilm 04 agricultural and veterinary sciences biology.organism_classification Thailand Galleria mellonella 030104 developmental biology Vibrio Infections 040102 fisheries 0401 agriculture forestry and fisheries Bacteria Bacterial Outer Membrane Proteins |
| Zdroj: | Journal of fish diseasesREFERENCES. 43(2) |
| ISSN: | 1365-2761 |
| Popis: | Vibrio alginolyticus is one of the most serious causative agents of diseases in cultured marine fish and shellfish. However, the characteristics of virulence factors in pathogenic V. alginolyticus are poorly known. To gain insight into fish diseases caused by V. alginolyticus, we carried out two-dimensional gel electrophoresis (2-DE) combined with MALDI-TOF mass spectrometry to identify uniquely expressed proteins in the disease-causing V. alginolyticus. V. alginolyticus strains were isolated from marine environments and diseased fish obtained from southern Thailand. We identified seven unique proteins in the disease-causing V. alginolyticus strain. Among those, the outer membrane protein A (OmpA) had the strongest expression. Therefore, the function of this protein was further analysed. To investigate the role of OmpA protein, an in-frame deletion mutant of ompA was constructed using the homologous recombination method. Although the ompA mutant V. alginolyticus strain (ΔompA) grew normally, the mutant exhibited a significant defect in the swarming ability and the biofilm formation. Furthermore, Galleria mellonella larvae injected with the mutant bacteria had a significantly greater survival percentage than those injected with the wild-type strain, demonstrating that OmpA protein is required for the pathogenicity of V. alginolyticus. Together, this study suggests a potential target for vaccine development against pathogenic V. alginolyticus strain. |
| Databáze: | OpenAIRE |
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