Characterization and Inductive Expression Analysis of Interferon Gamma-Related Gene in the Indian Major Carp, Rohu (Labeo rohita)
| Autor: | Pallipuram Jayasankar, Mrinal Samanta, Saswati S. Lenka, Surajit Das, Madhubanti Basu, Banikalyan Swain |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Fish Proteins
Lipopolysaccharides Carps DNA Complementary Blotting Western Molecular Sequence Data Real-Time Polymerase Chain Reaction Fish Diseases Interferon-gamma Exon Gene expression Tumor Cells Cultured Genetics medicine Animals Interferon gamma Amino Acid Sequence RNA Messenger Cloning Molecular Carp Molecular Biology Gene Receptors Interferon Regulation of gene expression Bacteria Base Sequence Sequence Homology Amino Acid biology Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Bacterial Infections Cell Biology General Medicine Anatomy biology.organism_classification Molecular biology Grass carp Gene expression profiling Gene Expression Regulation medicine.drug |
| Zdroj: | DNA and Cell Biology. 34:367-378 |
| ISSN: | 1557-7430 1044-5498 |
| DOI: | 10.1089/dna.2014.2656 |
| Popis: | Interferon gamma (IFN-γ) is one of the key cytokines that plays a major role against viral and intracellular bacterial infection. In addition to the IFN-γ gene, teleost fish possess a second copy known as IFN-γ-related (IFN-γrel) gene. This report describes structural and functional properties of IFN-γrel gene in the Indian major carp, rohu (Labeo rohita), a commercially important freshwater fish species in the Indian subcontinent. The rohu IFN-γrel gene consisted of four exons with three intervening introns and phylogenetically closely related to grass carp. The full-length IFN-γrel cDNA comprised 927 bp nucleotides with a single open reading frame of 504 bp, encoding 167 amino acids (aa) polypeptide with a signal peptide of 24 aa. The mature rohu IFN-γrel protein was 143 aa with a predicted molecular weight of 16.85 kDa. Basal expression analysis of IFN-γrel showed its wide range of expression in all examined tissues: The highest was in the skin and the lowest was in the liver. In response to LPS, poly I:C, iE-DAP, muramyl dipeptide stimulations, and bacterial infections, IFN-γrel gene expression was significantly (p |
| Databáze: | OpenAIRE |
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