Persistence of viral RNA in 2 rat strains differing in susceptibility to postbronchiolitis airway dysfunction
| Autor: | Louis A. Rosenthal, Anne G. Mosser, Robert F. Lemanske, Kristine Grindle, James E. Gern, Lance D. Mikus, Ronald L. Sorkness |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Male
viruses Immunology Orthomyxoviridae Viral Plaque Assay Respirovirus Infections Sendai virus Virus Immune system Rats Inbred BN medicine Animals Bronchiolitis Viral Immunology and Allergy Lung biology Respiration Disorders biology.organism_classification medicine.disease Rats Inbred F344 Rats Virus Latency medicine.anatomical_structure Bronchiolitis RNA Viral Disease Susceptibility Viral disease Lymph |
| Zdroj: | Journal of Allergy and Clinical Immunology. 110:607-609 |
| ISSN: | 0091-6749 |
| DOI: | 10.1067/mai.2002.128241 |
| Popis: | After viral bronchiolitis at an early age, a chronic asthma-like syndrome develops in BN, but not F344, rats. We hypothesized that the BN strain is less effective at clearing virus from the involved tissues. Weanling BN and F344 rats were inoculated with Sendai virus, and lung and peribronchial lymph nodes were harvested from each strain at 5 to 84 days after infection; control tissues were obtained from noninfected rats. Lung viral titers were similar for the 2 strains, with no infectious virus detectable by day 10. However, viral RNA was detected consistently by means of RT-PCR analyses in lungs and lymph nodes of both strains from days 10 to 27 and was still present at day 84 in some of the tissues from each strain. In contrast, there were strain-related differences in immune responses because IL-13 levels remained increased in the lung secretions of BN rats at 4 weeks after inoculation. Thus although Sendai virus could persist for at least 3 months after an acute infection in rats, this did not differ with strain. The persistent increase in IL-13 suggests instead that the strain-related variability in virus-associated airway pathology might be determined by the host response to infection rather than by the intensity or duration of infection. (J Allergy Clin Immunol 2002;110:607-9.) |
| Databáze: | OpenAIRE |
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