Characterization of the Relationship Between the Expression of Aspartate β-Hydroxylase and the Pathological Characteristics of Breast Cancer

Autor: Yanan Zhang, Yimeng Gao, Haitao Xie, Yingxue Li, Xuedong Zhang
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Leptin
Caveolin 1
Datasets as Topic
Muscle Proteins
030204 cardiovascular system & hematology
Mixed Function Oxygenases
0302 clinical medicine
Gene Regulatory Networks
Lymph node
biology
medicine.diagnostic_test
Carcinoma
Ductal
Breast
General Medicine
Middle Aged
Prognosis
Gene Expression Regulation
Neoplastic
medicine.anatomical_structure
030220 oncology & carcinogenesis
Disease Progression
Immunohistochemistry
Biological Markers
Female
Metabolic Networks and Pathways
Adult
Peroxisome proliferator-activated receptor gamma
Perilipin-1
Breast Neoplasms
Dioxygenases
03 medical and health sciences
Breast cancer
Atlases as Topic
Western blot
Clinical Research
medicine
Humans
RNA
Messenger
KEGG
Gene
Aged
Neoplasm Staging
Calcium-Binding Proteins
Membrane Proteins
Molecular Sequence Annotation
Lipase
medicine.disease
Survival Analysis
ASPH
PPAR gamma
Gene Ontology
Cancer research
biology.protein
Acyltransferases
Zdroj: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
ISSN: 1643-3750
1234-1010
Popis: BACKGROUND This study aimed to investigate the relationship between the expression of aspartate b-hydroxylase (ASPH) and the molecular mechanisms of ASPH-related genes in breast cancer (BC). MATERIAL AND METHODS ASPH expression was determined by immunohistochemistry and western blot analysis in samples of BC tissues and adjacent normal tissues. ASPH mRNA expression data and their clinical significance in BC were retrieved from the Oncomine and GEPIA datasets. Enrichment analysis of genes coexpressed with ASPH and annotation of potential pathways were performed with Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene ontology (GO) analysis. Hub genes were shown in an ASPH coexpression gene-interaction network. The expression of the hub genes associated with patient survival were analyzed to determine the role of ASPH in the progression of BC. RESULTS ASPH levels were overexpressed in BC and correlated with cancer type, lymph node involvement, and TNM stage. Conversely, ASPH levels did not correlate with patient age, invasive carcinoma types, or molecular subtypes. Enrichment analysis showed the involvement of multiple pathways, including lipid metabolism and oxidation-reduction processes. Six hub genes, PPARG, LEP, PLIN1, AGPAT2, CAV1, and PNPLA2, were related to ASPH expression and had functional roles in the occurrence and progression of BC. CONCLUSIONS ASPH may be involved in the development of BC and may have utility as a prognostic biomarker in BC. The coexpression of ASPH-associated genes may also be beneficial in improving BC prognosis.
Databáze: OpenAIRE
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