CK2 activity is modulated by growth rate in Saccharomyces cerevisiae
| Autor: | Luca Brambilla, Maria Patrizia Schiappelli, Farida Tripodi, Marco Vanoni, Danilo Porro, Paola Coccetti, Lilia Alberghina, Oriano Marin, Claudia Cirulli, Veronica Reghellin |
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| Přispěvatelé: | Tripodi, F, Cirulli, C, Reghellin, V, Marin, O, Brambilla, L, Schiappelli, M, Porro, D, Vanoni, M, Alberghina, L, Coccetti, P |
| Rok vydání: | 2010 |
| Předmět: |
animal structures
Solid-phase synthesis Saccharomyces cerevisiae Biophysics Chemostat Biochemistry Saccharomyces cerevisiae Growth rate Carbon source Phosphorylation In vivo Protein kinase CK2 Enzyme kinetics Casein Kinase II Protein kinase A Molecular Biology Cell Nucleus biology Synthetic peptides fungi Cell Biology biology.organism_classification BIO/10 - BIOCHIMICA Sic1 Carbon Cell biology embryonic structures biology.protein Phosphorylation Intracellular |
| Zdroj: | Biochemical and Biophysical Research Communications. 398:44-50 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2010.06.028 |
| Popis: | CK2 is a highly conserved protein kinase controlling different cellular processes. It shows a higher activity in proliferating mammalian cells, in various types of cancer cell lines and tumors. The findings presented herein provide the first evidence of an in vivo modulation of CK2 activity, dependent on growth rate, in Saccharomyces cerevisiae. In fact, CK2 activity, assayed on nuclear extracts, is shown to increase in exponential growing batch cultures at faster growth rate, while localization of catalytic and regulatory subunits is not nutritionally modulated. Differences in intracellular CK2 activity of glucose- and ethanol-grown cells appear to depend on both increase in molecule number and kcat. Also in chemostat cultures nuclear CK2 activity is higher in faster growing cells providing the first unequivocal demonstration that growth rate itself can affect CK2 activity in a eukaryotic organism. |
| Databáze: | OpenAIRE |
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