Epitope Mapping of PMab-241, a Lymphatic Endothelial Cell-Specific Anti-Bear Podoplanin Monoclonal Antibody
| Autor: | Teizo Asano, Yoshimi Nakamura, Miyuki Yanaka, Yusuke Sayama, Yoshikazu Furusawa, Yu Komatsu, Junko Takei, Mika K. Kaneko, Saori Handa, Takuro Nakamura, Yukinari Kato, Mikiko Yanagawa, Masato Sano, Saki Okamoto |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Platelet Aggregation medicine.drug_class Immunology CHO Cells Monoclonal antibody Epitope Epitopes 03 medical and health sciences Cricetulus 0302 clinical medicine Antibody Specificity Cricetinae medicine Animals Humans Immunology and Allergy Autoantibodies Membrane Glycoproteins 030102 biochemistry & molecular biology Podocytes Chemistry Antibodies Monoclonal Endothelial Cells Molecular biology Endothelial stem cell Lymphatic system Epitope mapping Podoplanin 030220 oncology & carcinogenesis Epitope Mapping Ursidae Immunostaining |
| Zdroj: | Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. 39:77-81 |
| ISSN: | 2167-9436 |
| DOI: | 10.1089/mab.2020.0004 |
| Popis: | Anti-bear podoplanin (bPDPN) monoclonal antibodies (mAbs), including PMab-247 and PMab-241, have been previously established. Although PMab-247 has shown positive immunostaining for lymphatic endothelial cells (LECs), type I alveolar cells of the lung, and podocytes of the kidney, PMab-241 stains LECs but does not react with lung type I alveolar cells. PDPN possesses three platelet aggregation-stimulating (PLAG) domains (PLAG1, PLAG2, and PLAG3) and the PLAG-like domain (PLD). The binding epitope of PMab-247 was previously determined to include bPDPN residues Asp76, Arg78, Glu80, and Arg82. Among these, Glu80 and Arg82 are included in PLD of bPDPN. The purpose of this study is to determine the binding epitope of PMab-241 and to clarify the difference between these two anti-bPDPN mAbs. Analysis of bPDPN deletion mutants revealed that the N-terminus of the PMab-241 epitope exists between amino acids (aa) 75 and 80 of bPDPN. In addition, analysis of bPDPN point mutants demonstrated that the critical epitope of PMab-241 includes Thr75, Asp76, and Arg78 of bPDPN. The binding epitopes of PMab-241 and PMab-247 seem to overlap, but this slight difference may be sufficient to provide the specificity of PMab-241 to discriminate LECs from type I alveolar cells of the lung. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|