Bone tissue engineering using polyetherketoneketone scaffolds combined with autologous mesenchymal stem cells in a sheep calvarial defect model
| Autor: | Ali Modabber, Carina Adamzyk, Sabine Neuss, Mareike Hoss, Rene Tolba, Felix Gremse, Paul Kachel, Bernd Lethaus, Frank Hölzle |
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| Přispěvatelé: | MUMC+: MA Mondzorg Kaak Aangezicht Chirurgie (9), RS: FHML non-thematic output, RS: GROW - R3 - Innovative Cancer Diagnostics & Therapy |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Scaffold Bone Regeneration Polymers Cell Bone healing Mesenchymal Stem Cell Transplantation Bone tissue engineering Biomaterials 03 medical and health sciences chemistry.chemical_compound Benzophenones Tissue engineering Osseointegration Bone reconstruction medicine Animals Humans Calvarial defect Sheep Tissue Engineering Tissue Scaffolds business.industry Mesenchymal stem cell Skull Polyetherketoneketone Anatomy Disease Models Animal 030104 developmental biology medicine.anatomical_structure Otorhinolaryngology chemistry Models Animal Mesenchymal stem cells Surgery Oral Surgery business Biomedical engineering |
| Zdroj: | Journal of Cranio-Maxillofacial Surgery, 44(8), 985-994. Churchill Livingstone |
| ISSN: | 1878-4119 1010-5182 |
| Popis: | Polyetherketoneketone (PEKK) a high performance thermoplastic polymer that is FDA-approved for cranio- and maxillo-facial as well as spineal surgery. We studied the viability, growth and osteogenic differentiation of bone marrow-derived human and sheep mesenchymal stem cells (MSC) in combination with a 3D scaffold made of PEKK using different cell-based assays. To investigate if autologous MSC, either undifferentiated or osteogenically pre-differentiated, augmented bone formation after implantation, we implanted cell-seeded 3D PEKK scaffolds into calvarial defects in sheep for 12 weeks. The volume and quality of newly formed bone were investigated using micro-computer tomography (micro-CT) and histological stainings. Our results show that the 3D PEKK scaffolds were cyto- and bio-compatible. They allowed for adherence, growth and osteogenic differentiation of human and ovine MSC. However, bone healing seemed unaffected by whether the scaffolds were seeded with MSC. Considerable amounts of newly formed bone were found in all PEKK treated groups, but a fibrous capsule was formed around the implants regardless of cell seeding with MSC. European Association for Cranio-Maxillo-Facial Surgery. |
| Databáze: | OpenAIRE |
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