Photobiomodulation of fresh bone marrow aspirate for regenerative therapy
| Autor: | Bianca Eduarda de Lima Neves, Liziana Jancos Calles, C.S. Santinoni, Thaís Sanches Leite Patara, Marcela Lucio Caldeira, Luciana Prado Maia, Nathália Laís Farias, Christine Men Martins |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Low-level laser therapy
Células madre Terapia a laser de baixa intensidade Cell Medula óssea Bone healing Stem cells Supervivencia celular Cell survival Andrology chemistry.chemical_compound medicine Terapia con láser de bajo nivel Bone marrow Viability assay Incubation General Environmental Science Mesenchymal stem cell Médula ósea Rats Ratas medicine.anatomical_structure chemistry Distilled water Cell culture General Earth and Planetary Sciences Trypan blue Células-tronco Sobrevivente de célula Ratos |
| Zdroj: | Research, Society and Development; Vol. 10 No. 11; e140101119545 Research, Society and Development; Vol. 10 Núm. 11; e140101119545 Research, Society and Development; v. 10 n. 11; e140101119545 Research, Society and Development Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
| ISSN: | 2525-3409 |
| Popis: | Use of mesenchymal stem cells and low-level laser therapy (LLLT) have been widely studied to promote bone healing. evaluate effect of photobiomodulation on total number of cells (TNC) and cell viability (CV) of fresh bone marrow aspirate (BMA). Femur BMA from 10 adult rats was collected and a cell concentration of 1x107 cell/mL was obtained. Cell suspension was deposited on 96 well cell culture plates and distributed in groups: 1) RPMI, positive control; 2) Distilled Water, negative control; 3) Red Laser (RL); 4) Infrared Laser (IRL). Groups RL and IRL received LLLT application right after incubation. Cells were incubated for 24 h. TNC and CV were assessed through trypan blue assay after 1, 3, 6, 10 and 24 h of incubation. Data distribution was verified by Shapiro-Wilk test. Kruskal-Wallis test was used for intergroup and intragroup comparisons (p |
| Databáze: | OpenAIRE |
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