Inhibition study of rhodanese by means of electrophoretically mediated microanalysis
| Autor: | Son̆a Nováková, Zdenĕk Glatz, Magdaléna Telnarová |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Thiosulfate
chemistry.chemical_classification Electrophoresis 0303 health sciences Chromatography Cyanide 010401 analytical chemistry Organic Chemistry General Medicine Electrolyte Rhodanese 01 natural sciences Biochemistry Microanalysis Thiosulfate Sulfurtransferase 0104 chemical sciences Analytical Chemistry Substrate Specificity 03 medical and health sciences chemistry.chemical_compound Capillary electrophoresis Enzyme chemistry Uncompetitive inhibitor 030304 developmental biology |
| Zdroj: | Journal of chromatography. A. 990(1-2) |
| ISSN: | 0021-9673 |
| Popis: | A combination of the electrophoretically mediated microanalysis methodology with a partial filling technique was applied for the inhibition study of bovine liver rhodanese by 2-oxoglutarate. In this set-up, part of the capillary is filled with the best buffer for the enzymatic reaction, while the rest of the capillary is filled with the optimal background electrolyte for separation of substrates and products. The estimated value of K1 for 2-oxoglutarate was 3.62 x 10(-4) +/- 1.43 x 10(-4) M with respect to cyanide and 1.40 x 10(-3) +/- 1.60 x 10(-4) M with respect to thiosulfate. In addition, the type of inhibition was also evaluated. The findings of 2-oxoglutarate as the competitive inhibitor with respect to cyanide and as the uncompetitive inhibitor with respect to thiosulfate are in accordance with previous literature data. |
| Databáze: | OpenAIRE |
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