COX‐1+/−COX‐2−/− genotype in mice is associated with shortened time to carotid artery occlusion through increased PAI‐1
| Autor: | L. He, Terrence E. Riehl, S. Greco, Douglas M. Tollefsen, L. Zheng, William F. Stenson |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty Genotype medicine.medical_treatment Prostacyclin Article Mice Thromboxane A2 chemistry.chemical_compound Internal medicine Plasminogen Activator Inhibitor 1 Fibrinolysis Euglobulin lysis time medicine Animals Carotid Stenosis Artery occlusion DNA Primers Mice Knockout Reverse Transcriptase Polymerase Chain Reaction business.industry Carotid Artery Thrombosis Hematology Mice Inbred C57BL Disease Models Animal Endocrinology chemistry Cyclooxygenase 2 Carotid artery occlusion Plasminogen activator inhibitor-1 Immunology Cyclooxygenase 1 Female business medicine.drug |
| Zdroj: | Journal of Thrombosis and Haemostasis. 9:350-360 |
| ISSN: | 1538-7836 |
| DOI: | 10.1111/j.1538-7836.2010.04156.x |
| Popis: | Summary. Background: We found a high incidence of thrombotic deaths in COX-1+/−COX-2−/− mice and sought to define the mechanism of these events. The cyclooxygenase products thromboxane A2 and prostacyclin are important in the regulation of coagulation but their role in fibrinolysis is largely unexplored. PAI-1 blocks fibrinolysis by inhibiting plasminogen activator. Aim: Our objective was to explain the mechanism of increased thrombosis associated with the COX-1+/−COX-2−/− genotype. Methods: Carotid artery occlusion times were measured after photochemical injury. PAI-1 levels were measured in the plasma by ELISA. PAI-1 levels in the aorta were measured by RT-PCR and Western blotting. Urinary metabolites of Thromboxane A2 and prostacyclin were measured by ELISA. Results: The COX-1+/−COX-2−/− genotype is associated with a decreased time to occlusion in the carotid artery thrombosis model (30 ± 5 minutes vs 60 ± minutes in wild type, p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text