Anti‐ Candida , docking studies, and in vitro metabolism‐mediated cytotoxicity evaluation of Eugenol derivatives

Autor: Jessyca Aparecida Paes, Dutra, Sarah Canal, Maximino, Rita de Cássia Ribeiro, Gonçalves, Pedro Alves Bezerra, Morais, William Cezar, de Lima Silva, Ricardo Pereira, Rodrigues, Álvaro Cunha, Neto, Valdemar Lacerda, Júnior, Warley, de Souza Borges, Rodrigo Rezende, Kitagawa
Rok vydání: 2022
Předmět:
Zdroj: Chemical Biology & Drug Design. 101:350-363
ISSN: 1747-0285
1747-0277
DOI: 10.1111/cbdd.14131
Popis: The high morbidity and mortality rates of Candida infections, especially among immunocompromised patients, are related to the increased resistance rate of these species and the limited therapeutic arsenal. In this context, we evaluated the anti-Candida potential and the cytotoxic profile of eugenol derivatives. Anti-Candida activity was evaluated on C. albicans and C. parapsilosis strains by minimum inhibitory concentration (MIC), scanning electron microscopy (SEM), and molecular docking calculations at the site of the enzyme lanosterol-14-α-demethylase active site, responsible for ergosterol formation. The cytotoxic profile was evaluated in HepG2 cells, in the presence and absence of the metabolizing system (S9 system). The results indicated compounds 1b and 1d as the most active ones. The compounds have anti-Candida activity against both strains with MIC ranging from 50 to 100 μg ml
Databáze: OpenAIRE
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