Analysis of Poly(ADP-Ribose) Polymerases in Arabidopsis Telomere Biology
| Autor: | Jennifer M. Townley, Dorothy E. Shippen, Kara A. Boltz, Madhu Jasti |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
0106 biological sciences
Telomerase Mutant Arabidopsis lcsh:Medicine Plant Science Plant Genetics 01 natural sciences Biochemistry chemistry.chemical_compound PARP1 Gene Expression Regulation Plant Molecular Cell Biology Plant Genomics Enzyme Inhibitors lcsh:Science Telomere-binding protein 0303 health sciences Multidisciplinary biology Chromosome Biology Genomics Telomere Nucleic acids Isoenzymes Telomeres Benzamides Poly(ADP-ribose) Polymerases Research Article Chromosome Structure and Function Zeocin DNA repair Arabidopsis Thaliana Poly(ADP-ribose) Polymerase Inhibitors Molecular Genetics 03 medical and health sciences Bleomycin Model Organisms Plant and Algal Models Genetics RNA Messenger Biology 030304 developmental biology lcsh:R Telomere Homeostasis DNA biology.organism_classification Molecular biology chemistry Mutation lcsh:Q Plant Biotechnology Gene Function Gene Deletion 010606 plant biology & botany |
| Zdroj: | PLoS ONE PLoS ONE, Vol 9, Iss 2, p e88872 (2014) |
| ISSN: | 1932-6203 |
| Popis: | Maintaining the length of the telomere tract at chromosome ends is a complex process vital to normal cell division. Telomere length is controlled through the action of telomerase as well as a cadre of telomere-associated proteins that facilitate replication of the chromosome end and protect it from eliciting a DNA damage response. In vertebrates, multiple poly(ADP-ribose) polymerases (PARPs) have been implicated in the regulation of telomere length, telomerase activity and chromosome end protection. Here we investigate the role of PARPs in plant telomere biology. We analyzed Arabidopsis thaliana mutants null for PARP1 and PARP2 as well as plants treated with the PARP competitive inhibitor 3-AB. Plants deficient in PARP were hypersensitive to genotoxic stress, and expression of PARP1 and PARP2 mRNA was elevated in response to MMS or zeocin treatment or by the loss of telomerase. Additionally, PARP1 mRNA was induced in parp2 mutants, and conversely, PARP2 mRNA was induced in parp1 mutants. PARP3 mRNA, by contrast, was elevated in both parp1 and parp2 mutants, but not in seedlings treated with 3-AB or zeocin. PARP mutants and 3-AB treated plants displayed robust telomerase activity, no significant changes in telomere length, and no end-to-end chromosome fusions. Although there remains a possibility that PARPs play a role in Arabidopsis telomere biology, these findings argue that the contribution is a minor one. |
| Databáze: | OpenAIRE |
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