Systemic DNA immunization against ovine lentivirus using particle-mediated epidermal delivery and modified vaccinia Ankara encoding the gag and/or env genes
| Autor: | Tom N. McNeilly, Maurizio Mazzei, Craig Watkins, X. de Andrés, H. Arnarson, H. Crespo, C. Huard, Idoia Glaria, Valgerdur Andrésdóttir, Sigurbjörg Torsteinsdóttir, Lluís Luján, Gordon D. Harkiss, Damián F. de Andrés, Cyril Barbezange, Francesco Tolari, C. Liu, I. de Blas, H. Niesalla, Ml Carrozza, G. Pétursson, Christophe Fraisier, Marta M. Pérez, Marie Suzan-Monti, Beatriz Amorena, Juan José Badiola, E. Biescas, Cristina Solano, Ramsés Reina, Sergio Rosati, Barbara Blacklaws, Michel Pépin, D. J. Shaw, P. Bandecchi |
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| Přispěvatelé: | CSIC-Public University of Navarra, University of Cambridge [UK] (CAM), Aix-Marseille Université - Faculté de médecine (AMU MED), Aix Marseille Université (AMU), University of Iceland [Reykjavik], University of Zaragoza - Universidad de Zaragoza [Zaragoza], University of Pisa - Università di Pisa, University of Edinburgh, University of Turin, Laboratoire d'études et de recherches sur les petits ruminants et les abeilles, Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Universidad Pública de Navarra [Espagne] = Public University of Navarra (UPNA), Università degli studi di Torino = University of Turin (UNITO), Laboratoire d'études et de recherches sur les petits ruminants et les abeilles (LERPRA), European Commission, Ministerio de Educación y Ciencia (España), Comisión Interministerial de Ciencia y Tecnología, CICYT (España) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Male
Modified vaccinia Ankara MESH: Biolistics Visna-maedi virus T-Lymphocytes viruses DNA vaccination Antibodies Viral env/*genetics/immunology Genes Gene gun MESH: Proviruses Proviruses MESH: Vaccinia virus Vaccines DNA MESH: Animals 1UVIROLOGIE HAFSSA AUTRE Animals Antibodies 0303 health sciences biology Pneumonia Progressive Interstitial of Sheep MESH: Genes env MESH: Pneumonia Progressive Interstitial of Sheep Provirus Genes gag 3. Good health MESH: Visna-maedi virus Infectious Diseases [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Molecular Medicine MESH: Virion MESH: Immunization Female Antibody Viral/blood *Biolistics Epidermis/virology Female Genes Progressive Interstitial MESH: Sheep MESH: Genes gag Vaccinia virus Genes env Virus Viral vector 03 medical and health sciences MESH: Viral Vaccines Animals Immune response Particle-mediated epidermal bombardment 030304 developmental biology Sheep General Veterinary General Immunology and Microbiology 030306 microbiology Lentivirus Public Health Environmental and Occupational Health Virion Viral Vaccines DNA/administration & dosage/genetics/immunology Vaccinia virus/*genetics/immunology Viral Vaccines/administration & dosage/genetics/immunology Virion/genetics/immunology Visna-maedi virus Group-specific antigen Biolistics biochemical phenomena metabolism and nutrition Virology Molecular biology MESH: Male MESH: Vaccines DNA MESH: T-Lymphocytes gag/*genetics/immunology Immunization Male Pneumonia biology.protein Immunization Epidermis MESH: Epidermis of Sheep/*prevention & control/virology Proviruses/isolation & purification Sheep T-Lymphocytes/immunology *Vaccines MESH: Female MESH: Antibodies Viral |
| Zdroj: | Vaccine Vaccine, Elsevier, 2009, 27 (2), pp.260-9. ⟨10.1016/j.vaccine.2008.10.042⟩ Vaccine, 2009, 27 (2), pp.260-9. ⟨10.1016/j.vaccine.2008.10.042⟩ Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2008.10.042⟩ |
| Popis: | Niesalla, H. et al. To determine whether systemic immunization with plasmid DNA and virus vector against visna/maedi virus (VMV) would induce protective immune responses, sheep were immunized with VMV gag and/or env sequences using particle-mediated epidermal bombardment and injection of recombinant modified vaccinia Ankara. The results showed that immunization induced both humoral and cell-mediated responses prior to and after virus challenge. The vaccination protocol did not prevent infection, but immunization with the gag gene or a combination of gag and env genes resulted in significantly reduced provirus loads in blood and mediastinal lymph node, respectively. Provirus loads in lung and draining lymph node were unaffected, but p25 expression was undetectable in lungs of animals immunized with a combination of gag and env genes. Analysis of target tissues for lesions at post-mortem showed that immunization with the env gene caused a significant increase in lesion score, while the gag gene or a combination of gag and env genes had no effect. Inclusion of the ovine interferon-γ gene in the initial priming mixture had minimal effect on immune responses, provirus load, or lesion development, although it resulted in a decreased p25 expression in the lung. The results thus show that systemic immunization with gag or a combination of gag and env genes reduces provirus load in blood and lymphoid tissue, respectively whereas env immunization has no effect on provirus load but increased lesion development. © 2008 Elsevier Ltd. All rights reserved. This study was supported by grants from European Union (QLK2-CT-2002-00617) and Spanish CICYT (AGL2003-08977-C03-01 and AGL2007-66874-C04). Ramsés Reina and Ximena de Andrés were supported by a fellowship FPI from the Spanish Ministry of Science and Education. Tom McNeilly was supported by a PhD studentship from the Royal (Dick) College of Veterinary Studies, University of Edinburgh. |
| Databáze: | OpenAIRE |
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