Preferential effect of synchrotron microbeam radiation therapy on intracerebral 9L gliosarcoma vascular networks
| Autor: | Emmanuel L. Barbier, Audrey Bouchet, Enam A. Khalil, Cécile Maisin, Géraldine Le Duc, Chantal Rémy, Jean A. Laissue, Elke Bräuer-Krisch, E.A. Siegbahn, Alberto Bravin, Benjamin Lemasson, Raphaël Serduc, Luc Renaud, Cathy Poillot |
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| Přispěvatelé: | Bouchet, A, Lemasson, B, Le Duc, G, Maisin, C, Brauer-Krisch, E, Siegbahn Erik, A, Renaud, L, Khalil, E, Remy, C, Poillot, C, Bravin, A, Laissue Jean, A, Barbier Emmanuel, L, Serduc, R, European Synchrotron Radiation Facility (ESRF), Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Oncodesign [Dijon], Department of Medical Physics, Karolinska Hospital, Centre de recherche cerveau et cognition (CERCO), Institut des sciences du cerveau de Toulouse. (ISCT), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Faculty of Pharmacy, The University of Jordan (JU), Oncology - Pathology - Anatomy, Institute of Pathology-University of Bern, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Dojat, Michel |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Vascular Endothelial Growth Factor A
Cancer Research Pathology MESH: Tumor Burden MESH: Brain Edema Synchrotron Microbeam radiation therapy/intracerebral 9L gliosarcoma/tumor vasculature Synchrotron Microbeam radiation therapy medicine.medical_treatment Brain Edema Radiation Tolerance MESH: Cerebral Veins 030218 nuclear medicine & medical imaging MESH: Magnetic Resonance Imaging chemistry.chemical_compound 0302 clinical medicine MESH: Animals MESH: Radiotherapy Dosage MESH: Cerebral Arteries Radiation MESH: Radiation Tolerance Brain Neoplasms Brain Radiotherapy Dosage MESH: Cerebrovascular Circulation Magnetic Resonance Imaging Tumor Burden Vascular endothelial growth factor medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Cerebrovascular Circulation Circulatory system MESH: Brain Neoplasms MESH: Synchrotrons Immunohistochemistry [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] Sarcoma Monte Carlo Method Blood vessel medicine.medical_specialty Gliosarcoma Endothelium MESH: Rats FIS/07 - FISICA APPLICATA (A BENI CULTURALI AMBIENTALI BIOLOGIA E MEDICINA) MESH: Monte Carlo Method Capillary Permeability 03 medical and health sciences MESH: Brain Intracerebral 9L gliosarcoma medicine Animals Radiology Nuclear Medicine and imaging [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] business.industry MESH: Rats Inbred F344 MESH: Vascular Endothelial Growth Factor A MESH: Capillary Permeability Cerebral Arteries medicine.disease Cerebral Veins Rats Inbred F344 Rats Radiation therapy tumor vasculature chemistry MESH: Gliosarcoma business Nuclear medicine Synchrotrons |
| Zdroj: | International Journal of Radiation Oncology-Biology-Physics International Journal of Radiation Oncology-Biology-Physics, Elsevier, 2010, 78 (5), pp.1503-12. ⟨10.1016/j.ijrobp.2010.06.021⟩ International Journal of Radiation Oncology, Biology, Physics International Journal of Radiation Oncology, Biology, Physics, 2010, 78 (5), pp.1503-12. ⟨10.1016/j.ijrobp.2010.06.021⟩ |
| ISSN: | 0360-3016 1879-355X |
| DOI: | 10.1016/j.ijrobp.2010.06.021⟩ |
| Popis: | International audience; PURPOSE: Synchrotron microbeam radiation therapy (MRT) relies on spatial fractionation of the incident photon beam into parallel micron-wide beams. Our aim was to analyze the effects of MRT on normal brain and 9L gliosarcoma tissues, particularly on blood vessels. METHODS AND MATERIALS: Responses to MRT (two arrays, one lateral, one anteroposterior (2 × 400 Gy), intersecting orthogonally in the tumor region) were studied during 6 weeks using MRI, immunohistochemistry, and vascular endothelial growth factor Western blot. RESULTS: MRT increased the median survival time of irradiated rats (×3.25), significantly increased blood vessel permeability, and inhibited tumor growth; a cytotoxic effect on 9L cells was detected 5 days after irradiation. Significant decreases in tumoral blood volume fraction and vessel diameter were measured from 8 days after irradiation, due to loss of endothelial cells in tumors as detected by immunochemistry. Edema was observed in the normal brain exposed to both crossfired arrays about 6 weeks after irradiation. This edema was associated with changes in blood vessel morphology and an overexpression of vascular endothelial growth factor. Conversely, vascular parameters and vessel morphology in brain regions exposed to one of the two arrays were not damaged, and there was no loss of vascular endothelia. CONCLUSIONS: We show for the first time that preferential damage of MRT to tumor vessels versus preservation of radioresistant normal brain vessels contributes to the efficient palliation of 9L gliosarcomas in rats. Molecular pathways of repair mechanisms in normal and tumoral vascular networks after MRT may be essential for the improvement of such differential effects on the vasculature. |
| Databáze: | OpenAIRE |
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