Clofibrate-induced alterations in serum protein patterns
| Autor: | Peter G. Canonico, Eric L. Henriksen, Michael C. Powanda, Eleanor Ayala |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Blood Glucose
Male Globulin Biochemistry chemistry.chemical_compound medicine Animals Clofibrate Polyacrylamide gel electrophoresis Triglycerides Pharmacology Chromatography biology Chemistry Haptoglobin Albumin Blood Proteins Blood Protein Electrophoresis Trypsin Blood proteins Cellulose acetate Rats Cholesterol biology.protein RNA medicine.drug |
| Zdroj: | Biochemical Pharmacology. 25:785-788 |
| ISSN: | 0006-2952 |
| DOI: | 10.1016/0006-2952(76)90147-7 |
| Popis: | Clofibrate when added to the diet at 0.05 to 1.25% (w/w) not only causes an apparently dose-dependent decrease in serum cholesterol of rats but also markedly affects the plasma protein pattern. An increase in albumin is detectable by cellulose acetate strip electrophoresis, acrylamide gel electrophoresis, and by extraction of albumin from plasma. Cellulose acetate strip electrophoresis reveals a decrease in the α 2 -globulin fraction, while acrylamide gel electrophoresis indicates that there are manifold changes. Extractable seromucoid concentration declined from 440 mg/100 ml to 150 mg/ml as the dose of clofibrate increased. A concentration decrease in plasma glucose was also observed. Part of the decrement in seromucoid at low drug levels may be related to lessened haptoglobin concentration. The trypsin inhibitory capacity of the plasma was significantly decreased in what appeared to be a dose-dependent fashion. The decrease in seromucoid is consistent with the reduction in bound fraction of ribosomal RNA. Another explanation would appear to be required to explain the rise in albumin. |
| Databáze: | OpenAIRE |
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