Retinoids regulate the anterior expression boundaries of 5' Hoxb genes in posterior hindbrain
| Autor: | Frits Meijlink, Jacqueline Deschamps, Pierre Chambon, Karen Niederreither, Tony Oosterveen, Pascal Dollé |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Central Nervous System
5' Flanking Region Retinal dehydrogenase medicine.drug_class Embryonic Structures Hindbrain Biology Response Elements General Biochemistry Genetics and Molecular Biology Evolution Molecular Mice Retinoids Genes Reporter Morphogenesis medicine Animals Humans Transgenes Retinoid Molecular Biology Gene In Situ Hybridization Homeodomain Proteins General Immunology and Microbiology General Neuroscience Genes Homeobox Neural tube Gene Expression Regulation Developmental Retinal Dehydrogenase Articles Zebrafish Proteins Embryo Mammalian Spinal cord Aldehyde Oxidoreductases Molecular biology Embryonic stem cell Rhombencephalon medicine.anatomical_structure Multigene Family embryonic structures Signal transduction Signal Transduction |
| Zdroj: | The EMBO Journal. 22:262-269 |
| ISSN: | 1460-2075 |
| DOI: | 10.1093/emboj/cdg029 |
| Popis: | We describe the regulatory interactions that cause anterior extension of the mouse 5′ Hoxb expression domains from spinal cord levels to their definitive boundaries in the posterior hindbrain between embryonic day E10 and E11.5. This anterior expansion is retinoid dependent since it does not occur in mouse embryos deficient for the retinoic acid-synthesizing enzyme retinaldehyde dehydrogenase 2. A retinoic acid response element (RARE) was identified downstream of Hoxb5 and shown to be essential for expression of Hoxb5 and Hoxb8 reporter transgenes in the anterior neural tube. The spatio-temporal activity of this element overlaps with rostral extension of the expression domain of endogenous Hoxb5, Hoxb6 and Hoxb8 into the posterior hindbrain. The RARE and surrounding sequences are found at homologous positions in the human, mouse and zebrafish genome, which supports an evolutionarily conserved regulatory function. |
| Databáze: | OpenAIRE |
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