Cold tolerance of potato plants transformed with yeast invertase gene
| Autor: | Elena P. Sabel´nikova, T. I. Trunova, Alexander N. Deryaabin, Irena M. Dubinina, Elena A. Burakhanova, Maxim S. Sinkevich, Nina V. Astakhova |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Reporter gene
biology low-temperature tolerance fungi food and beverages Plant Science Genetically modified crops biochemical phenomena metabolism and nutrition transgenic plants biology.organism_classification Plant cell exoosmos of electrolytes lcsh:S1-972 Yeast invertase Invertase Biochemistry sugars Solanum Patatin lcsh:Agriculture (General) Sugar Agronomy and Crop Science Ecology Evolution Behavior and Systematics Solanum tuberosum |
| Zdroj: | Acta Agrobotanica, Vol 57, Iss 1-2, Pp 31-39 (2013) |
| Popis: | Our study was carried out with potato plants (Solanum tuberosun L.,cv. Désirée) transformed with the yeast invertase gene under the control of the B33 class I patatin promoter and with the proteinase inhibitor II leader peptide sequence providing for the apoplastic enzyme localization (B33-inv plants) and with the plants transformed with the reporter gene encoding bb-glucuronidase under the control of the 35S CaMV promoter (control plants). Exposure to 5°C during 6 days caused an increase in invertase activity and sugar content in B33-inv leaves in comparison with the control plants. Cell membranes of B33-inv plant cells showed greater cold tolerance under low temperature conditions than control plants that was recorded by electrolyte release. We supposed that higher cold tolerance of B33-inv plants was caused by stabilizing effect of sugar on the membranes, because B33-inv plants differ from the control plants in higher invertase activity, induced by expression of yeast invertase gene, and high content of sugars. |
| Databáze: | OpenAIRE |
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