P-glycoprotein is expressed in the mineralizing regions of the skeleton
| Autor: | S. Komiya, Henry J. Mankin, K. Dunussi, R. J. Arceci, D C Mangham, A. Cannon, Mark C. Gebhardt, R. L. Gendron |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Adolescent Endocrinology Diabetes and Metabolism Molecular Sequence Data Matrix (biology) Polymerase Chain Reaction Bone and Bones Calcification Physiologic Fetus Endocrinology Pregnancy Internal medicine medicine Animals Humans Orthopedics and Sports Medicine ATP Binding Cassette Transporter Subfamily B Member 1 Growth Plate RNA Messenger Child P-glycoprotein Messenger RNA Base Sequence biology Chemistry Osteoid Cartilage Infant Newborn Antibodies Monoclonal Infant Amplicon Immunohistochemistry Skeleton (computer programming) Cell biology Blotting Southern medicine.anatomical_structure Child Preschool Bone Trabeculae biology.protein Cattle Female |
| Zdroj: | Calcified Tissue International. 58:186-191 |
| ISSN: | 1432-0827 0171-967X |
| DOI: | 10.1007/bf02526885 |
| Popis: | Using oligonucleotide primers specific for the human MDR 1 gene, we were able to identify a specific amplicon using RT-PCR from total bovine growth plate chondrocyte RNA. The identification of MDR mRNA in growth plate chondrocytes led us to examine the precise distribution of MDR P-glycoprotein in bone and cartilage. We applied two monoclonal antibodies (C219 and C494) to human fetal, neonatal, and childhood growth plates and bone. In growth plates, P-glycoprotein was detected at high levels in a perilacunar distribution in the calcifying zone and at lower levels in hypertrophic, but not proliferative or reserve zone, chondrocytes. P-glycoprotein was also observed in perichondrial chondrocytes, in perivascular chondrocytes and matrix in the fetal cartilage anlage, and in osteoblasts and the surface osteoid matrix of newly formed bone trabeculae in the primary spongiosa. The recently described chloride channel of P-glycoprotein suggests a potential role of P-glycoprotein in growth plate chondrocyte hypertrophy. |
| Databáze: | OpenAIRE |
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