Forskolin-loaded human serum albumin nanoparticles and its biological importance
| Autor: | Sailaja Nakkka, Daniel Pushparaju Yeggoni, Rajagopal Subramanyam, Veerababu Nagati |
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| Rok vydání: | 2020 |
| Předmět: |
Forskolin
Binding Sites Circular Dichroism Colforsin Nanoparticle Serum Albumin Human General Medicine Human serum albumin chemistry.chemical_compound Spectrometry Fluorescence chemistry Structural Biology Transmission electron microscopy medicine Biophysics Humans Nanoparticles Thermodynamics Surface plasmon resonance Molecular Biology medicine.drug Protein Binding |
| DOI: | 10.6084/m9.figshare.8124173.v2 |
| Popis: | In this study, forskolin-loaded human serum albumin nanoparticles (FR-HSANPs) were successfully prepared by incorporation and affinity-binding methods. FR-HSANPs were characterized by transmission electron microscope that most of them are circular in shape and size is around 340 nm. The drug loading was more than 88% and further sustained release profiles were observed as it is 77.5% in 24 h time. Additionally, the cytotoxicity results with HepG2 cells indicated that FR-HSANPs showed significantly higher cytotoxicity and lower cell viability as compared to free forskolin (FR). Furthermore, to understand the binding mechanism of human serum albumin (HSA) with forskolin resulted from fluorescence quenching as a static mechanism and the binding constant is 6.26 ± 0.1 × 104 M−1, indicating a strong binding affinity. Further, association and dissociation kinetics of forskolin–HSA was calculated from surface plasmon resonance spectroscopy and the binding constant found to be Kforskolin = 3.4 ± 0.24 × 104 M−1 and also fast dissociation was observed. Further, we used circular dichroism and molecular dynamics simulations to elucidate the possible structural changes including local conformational changes and rigidity of the residues of both HSA and HSA–forskolin complexes. Communicated by Ramaswamy H. Sarma |
| Databáze: | OpenAIRE |
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