Inhibition of Proteasome Activity Impairs Centrosome-dependent Microtubule Nucleation and Organization
Autor: | Nabila Jabrane-Ferrat, Christine Didier, Andreas Merdes, Jean-Edouard Gairin |
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Přispěvatelé: | Laboratoire de Biologie Cellulaire et Moléculaire du Contrôle de la Prolifération (LBCMCP), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre de Biologie Intégrative (CBI), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Wellcome Trust Centre for Cell Biology, University of Edinburgh, Institut des Sciences et du Medicament Toulouse, Toulouse, France. (UMR2587-Pierre Fabre), PIERRE FABRE-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre de Physiopathologie Toulouse Purpan (CPTP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
Rok vydání: | 2008 |
Předmět: |
MESH: Protein Transport
MESH: Ubiquitin animal structures Centriole Chromosomal Proteins Non-Histone NEDD1 [SDV.CAN]Life Sciences [q-bio]/Cancer Centrosome cycle MESH: Tubulin Aster (cell biology) Biology Microtubules MESH: Centrosome Cell Line 03 medical and health sciences 0302 clinical medicine Tubulin MESH: Chromosomal Proteins Non-Histone Microtubule Humans Enzyme Inhibitors Molecular Biology Centrioles 030304 developmental biology Pericentriolar material Microtubule nucleation Centrosome 0303 health sciences MESH: Humans Ubiquitin MESH: Centrioles MESH: Microtubules MESH: Proteasome Endopeptidase Complex Articles Cell Biology MESH: Cell Line Cell biology MESH: Solubility Protein Transport Solubility MESH: Organelle Size MESH: Enzyme Inhibitors MESH: Protein Processing Post-Translational 030220 oncology & carcinogenesis Organelle Size Proteasome Inhibitors Protein Processing Post-Translational |
Zdroj: | Molecular Biology of the Cell Molecular Biology of the Cell, American Society for Cell Biology, 2008, 19 (3), pp.1220-9. ⟨10.1091/mbc.E06-12-1140⟩ |
ISSN: | 1939-4586 1059-1524 |
DOI: | 10.1091/mbc.e06-12-1140 |
Popis: | International audience; Centrosomes are dynamic organelles that consist of a pair of cylindrical centrioles, surrounded by pericentriolar material. The pericentriolar material contains factors that are involved in microtubule nucleation and organization, and its recruitment varies during the cell cycle. We report here that proteasome inhibition in HeLa cells induces the accumulation of several proteins at the pericentriolar material, including gamma-tubulin, GCP4, NEDD1, ninein, pericentrin, dynactin, and PCM-1. The effect of proteasome inhibition on centrosome proteins does not require intact microtubules and is reversed after removal of proteasome inhibitors. This accrual of centrosome proteins is paralleled by accumulation of ubiquitin in the same area and increased polyubiquitylation of nonsoluble gamma-tubulin. Cells that have accumulated centrosome proteins in response to proteasome inhibition are impaired in microtubule aster formation. Our data point toward a role of the proteasome in the turnover of centrosome proteins, to maintain proper centrosome function. |
Databáze: | OpenAIRE |
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