Inhibition of Proteasome Activity Impairs Centrosome-dependent Microtubule Nucleation and Organization

Autor: Nabila Jabrane-Ferrat, Christine Didier, Andreas Merdes, Jean-Edouard Gairin
Přispěvatelé: Laboratoire de Biologie Cellulaire et Moléculaire du Contrôle de la Prolifération (LBCMCP), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre de Biologie Intégrative (CBI), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Wellcome Trust Centre for Cell Biology, University of Edinburgh, Institut des Sciences et du Medicament Toulouse, Toulouse, France. (UMR2587-Pierre Fabre), PIERRE FABRE-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre de Physiopathologie Toulouse Purpan (CPTP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2008
Předmět:
MESH: Protein Transport
MESH: Ubiquitin
animal structures
Centriole
Chromosomal Proteins
Non-Histone

NEDD1
[SDV.CAN]Life Sciences [q-bio]/Cancer
Centrosome cycle
MESH: Tubulin
Aster (cell biology)
Biology
Microtubules
MESH: Centrosome
Cell Line
03 medical and health sciences
0302 clinical medicine
Tubulin
MESH: Chromosomal Proteins
Non-Histone

Microtubule
Humans
Enzyme Inhibitors
Molecular Biology
Centrioles
030304 developmental biology
Pericentriolar material
Microtubule nucleation
Centrosome
0303 health sciences
MESH: Humans
Ubiquitin
MESH: Centrioles
MESH: Microtubules
MESH: Proteasome Endopeptidase Complex
Articles
Cell Biology
MESH: Cell Line
Cell biology
MESH: Solubility
Protein Transport
Solubility
MESH: Organelle Size
MESH: Enzyme Inhibitors
MESH: Protein Processing
Post-Translational

030220 oncology & carcinogenesis
Organelle Size
Proteasome Inhibitors
Protein Processing
Post-Translational
Zdroj: Molecular Biology of the Cell
Molecular Biology of the Cell, American Society for Cell Biology, 2008, 19 (3), pp.1220-9. ⟨10.1091/mbc.E06-12-1140⟩
ISSN: 1939-4586
1059-1524
DOI: 10.1091/mbc.e06-12-1140
Popis: International audience; Centrosomes are dynamic organelles that consist of a pair of cylindrical centrioles, surrounded by pericentriolar material. The pericentriolar material contains factors that are involved in microtubule nucleation and organization, and its recruitment varies during the cell cycle. We report here that proteasome inhibition in HeLa cells induces the accumulation of several proteins at the pericentriolar material, including gamma-tubulin, GCP4, NEDD1, ninein, pericentrin, dynactin, and PCM-1. The effect of proteasome inhibition on centrosome proteins does not require intact microtubules and is reversed after removal of proteasome inhibitors. This accrual of centrosome proteins is paralleled by accumulation of ubiquitin in the same area and increased polyubiquitylation of nonsoluble gamma-tubulin. Cells that have accumulated centrosome proteins in response to proteasome inhibition are impaired in microtubule aster formation. Our data point toward a role of the proteasome in the turnover of centrosome proteins, to maintain proper centrosome function.
Databáze: OpenAIRE