Comparison Between Total IgG, C1q, and C3d Single Antigen Bead Assays in Detecting Class I Complement-Binding Anti-HLA Antibodies
| Autor: | Manish J. Gandhi, Laurie L. Wakefield, Byron H. Smith, Donald Mitema, Carrie A. Schinstock, M. Moreno Gonzales, Nicole Henderson, Mark D. Stegall, Steven R. DeGoey, Justin D. Kreuter |
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| Rok vydání: | 2017 |
| Předmět: |
Graft Rejection
chemical and pharmacologic phenomena Human leukocyte antigen Immunologic Tests 030230 surgery Sensitivity and Specificity Serology 03 medical and health sciences 0302 clinical medicine HLA Antigens Isoantibodies Transplantation Immunology Odds Ratio Humans Hla antibodies Single antigen bead Cytotoxicity Complement C1q Immunoassay Transplantation biology Chemistry Mean fluorescence intensity Kidney Transplantation Molecular biology Immunoglobulin G biology.protein 030211 gastroenterology & hepatology Surgery Antibody Protein Binding |
| Zdroj: | Transplantation Proceedings. 49:2031-2035 |
| ISSN: | 0041-1345 |
| Popis: | Complement-binding donor-specific antibodies (DSAs) are associated with antibody-mediated rejection and allograft loss. Novel single antigen bead (SAB) assays-that is, complement component 1q (C1q) and complement component 3d (C3d) assays-have been developed to specifically detect complement-binding DSA, but it remains unclear whether these assays have an improved ability to detect complement-binding DSA as compared with using the total IgG SAB assay with a high mean fluorescence intensity (MFI) cutoff. The aim of this study was to compare the ability of the total IgG, C1q, and C3d SAB assays in detecting complement-binding anti-HLA antibodies.Twenty sera known to have complement-binding anti-HLA antibodies (serologic class I HLA typing by complement-dependent cytotoxicity method) were tested with 3 different SAB assays: total IgG (undiluted and 1:8 dilution), C1q, and C3d. Serologic anti-HLA specificities were compared with those obtained by IgG, C1q, and C3d SAB assays.IgG SAB was more sensitive in detecting complement-binding antibodies (sensitivity 24 of 24 = 1, odds ratio infinity). Pearson correlation showed the association between (1) C1q and IgG SAB assays (cutoff C1q SAB 1000 MFI, cutoff IgG SAB 5000 MFI: r = 0.347, P .0001) and (2) C3d and IgG SAB assays (cutoff 500 MFI C3d SAB, 5000 MFI for IgG SAB: r = -0.173, P = .279).For class I anti-HLA antibodies, IgG SAB (cutoff MFI5000) was more sensitive in detecting complement-binding antibodies when compared with C1q and C3d SAB assays. |
| Databáze: | OpenAIRE |
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