Neonatal Avian Pneumovirus Infection in Commercial Turkeys
| Autor: | M. K. Njenga, Faris F. Jirjis, M. C. Kumar, Kakambi V. Nagaraja, Daniel P. Shaw, Sally Noll, Hyun-Jin Shin, David A. Halvorson |
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| Rok vydání: | 2002 |
| Předmět: |
Turkeys
Veterinary medicine animal diseases Virus isolation Enzyme-Linked Immunosorbent Assay Biology Turbinates Virus Disease Outbreaks Food Animals Animals Pneumovirus Infections Poultry Diseases Pneumovirus General Immunology and Microbiology Geographic area Reverse Transcriptase Polymerase Chain Reaction Age Factors Immunohistochemistry Virology Reverse transcriptase Avian pneumovirus Nasal discharge nervous system RNA Viral Animal Science and Zoology Flock |
| Zdroj: | Avian Diseases. 46:239-244 |
| ISSN: | 1938-4351 0005-2086 |
| DOI: | 10.1637/0005-2086(2002)046[0239:napiic]2.0.co;2 |
| Popis: | Eleven market turkey flocks developed a respiratory disease characterized by coughing, swollen sinuses and nasal discharge. These symptoms first appeared between 3 and 16 days of age. Avian pneumovirus (APV) RNA was detected by reverse transcriptase (RT)-polymerase chain reaction (PCR) in six of six flocks tested. APV was detected by immunohistochemistry in turbinates of three of three affected flocks tested. Virus isolation attempts were negative. Ten of 11 flocks became seropositive on the APV enzyme-linked immunosorbent assay. Five weeks prior to hatch of these affected market turkeys, several breeder flocks in one geographic area had developed clinical signs and experienced decline in egg production typical of APV infection. In two breeder flocks, acute and convalescent sera indicated APV infection during the period of declining egg production. Attempts to detect APV RNA by RT-PCR from choanal cleft swabs of newly hatched poults were successful. Attempts to isolate the virus from these PCR-positive samples were negative. |
| Databáze: | OpenAIRE |
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