Genotyping of Giardia duodenalis in vegetables cultivated with organic and chemical fertilizer from street markets and community vegetable gardens in a region of Southern Brazil
| Autor: | Katyelle Rafael, Renata Coltro Bezagio, Fernanda Ferreira Evangelista, Ana Lúcia Falavigna-Guilherme, Ariella Andrade Marchioro, Bruna Tiaki Tiyo, Cristiane Maria Colli |
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| Rok vydání: | 2017 |
| Předmět: |
Giardiasis
0301 basic medicine Genotype Urban Population 030231 tropical medicine 030106 microbiology Molecular Technique engineering.material Commercial kit Biology Polymerase Chain Reaction Food Supply 03 medical and health sciences 0302 clinical medicine Feeding behavior Vegetables Animals Humans Cooking Fertilizers Genotyping Commerce Public Health Environmental and Occupational Health Feeding Behavior Sequence Analysis DNA General Medicine DNA Protozoan DNA extraction Diet Horticulture Infectious Diseases Giardia duodenalis engineering Food Organic Parasitology Fertilizer Giardia lamblia Cellulose acetate membrane Gardens Brazil Polymorphism Restriction Fragment Length |
| Zdroj: | Transactions of The Royal Society of Tropical Medicine and Hygiene. 111:540-545 |
| ISSN: | 1878-3503 0035-9203 |
| DOI: | 10.1093/trstmh/try013 |
| Popis: | Background In order to investigate the occurrence of Giardia duodenalis and its genotypes in vegetables that are consumed raw, we analyzed samples cultivated with organic or chemical fertilizer, sold in street markets and from community vegetable gardens in an urban area located in Southern Brazil. Methods We analyzed 130 samples of vegetables such as crisp lettuce, regular lettuce, kale, chicory and rocket, from street markets, and 130 from community gardens. From each sample, 50 g were washed in Tween 80 solution (1%) and the solution obtained was filtered through a cellulose acetate membrane. The retained material was used for DNA extraction with the commercial kit Purelink®. GDH gene was amplified by semi-nested PCR using the GDHeF, GDHiR and GDHiF primers. Positive samples were genotyped using the PCR-RFLP technique with the restriction enzyme NlaIV. Results We obtained 7.3% (19/260) positive samples for G. duodenalis, both from street markets (10/130) and from community gardens (9/130), including organic and non-organic products. The assemblage AI was predominant, but assemblages B and E were also found. Conclusions The molecular technique revealed genotypes with zoonotic potential, evidencing the importance of investigating commercialized vegetables that are consumed raw and establishing a more rigid quality control. |
| Databáze: | OpenAIRE |
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