Antinociceptive activity of Calotropis procera latex in mice
| Autor: | Krishnamurti M. Carvalho, Samara G. Matos, Cleverson D.T. Freitas, Bruno A. Cardi, David N. Criddle, Márcio V. Ramos, Manoel Cláudio Azevedo Patrocínio, Paula Matias Soares, Ana Maria Sampaio Assreuy, Marcelo de Moraes Andrade, Silvane R. Lima, Silvânia Maria Mendes Vasconcelos |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Hot Temperature Latex Stereochemistry Narcotic Antagonists Analgesic (+)-Naloxone Pharmacognosy Pharmacology Mice Acetic acid chemistry.chemical_compound Calotropis procera Formaldehyde Drug Discovery Reaction Time Animals Hot plate test Acetic Acid Pain Measurement Plant Proteins Analgesics Dose-Response Relationship Drug biology Naloxone biology.organism_classification Dose–response relationship Calotropis Nociception chemistry |
| Zdroj: | Journal of Ethnopharmacology. 99:125-129 |
| ISSN: | 0378-8741 |
| Popis: | This work evaluated the antinociceptive effect of proteins from the Calotropis procera (Asclepiadaceae) latex using three different experimental models of nociception in mice. The latex protein fraction administered intraperitoneally in male mice at the doses of 12.5, 25 and 50 mg/kg showed the antinociceptive effect in a dose dependent manner compared to the respective controls in all assays. Inhibitions of the acetic acid-induced abdominal constrictions were observed at the doses of 12.5 (67.9%), 25 (85%) and 50 (99.5%) mg/kg compared to controls. Latex protein at the doses of 25 (39.8%; 42%) and 50 mg/kg (66.6%; 99.3%) reduced the nociception produced by formalin in the 1st and 2nd phases, respectively, and this effect was not reversed by pretreatment with naloxone (1 mg/kg). In the hot plate test, an increase of the reaction time was observed only at 60 min after the treatment with latex at the doses of 25 (79.5%) and 50 (76.9%) mg/kg, compared to controls and naloxone was ineffective to reverse the effect. It was concluded that the protein fraction derived from the whole latex of Calotropis procera possesses antinociceptive activity, which is independent of the opioid system. |
| Databáze: | OpenAIRE |
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