Neutralising ability of Terminalia fagifolia extract (Combretaceae) against the in vitro neuromuscular effects of Bothrops jararacussu venom
| Autor: | José Carlos Cogo, Natália Tribuiani, Isadora Caruso Fontana Oliveira, Márcio Galdino dos Santos, Monique N. Santana, Jorge Amaral Filho, Marylu Oliveira Tavares, Magali Glauzer Silva, Yoko Oshima-Franco, Rafael Stuani Floriano, Karina Cogo-Müller |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Myotoxin Diaphragm Drug Evaluation Preclinical Saponin Venom Plant Science Jararacussu Pharmacology Biochemistry complex mixtures Analytical Chemistry Mice 03 medical and health sciences 0302 clinical medicine Crotalid Venoms Animals Bothrops Creatine Kinase chemistry.chemical_classification biology Plant Extracts Organic Chemistry Terminalia Anatomy biology.organism_classification Phrenic Nerve 030104 developmental biology Neuromuscular Agents chemistry Snake venom biology.protein Creatine kinase Chickens 030217 neurology & neurosurgery |
| DOI: | 10.6084/m9.figshare.4738321.v1 |
| Popis: | The ability of Terminalia fagifolia hydroalcoholic extract (Tf-HE) to neutralise the paralysis and myotoxicity induced by Bothrops jararacussu venom was assayed using mouse phrenic nerve-diaphragm (PND) preparation and two varieties of chick biventer cervicis (BC) preparations. Tf-HE 100 μg/mL and 500 μg/mL were tested against 40 and 200 μg of venom/mL in PND and BC preparations, respectively, using pre- and post-venom incubation treatments. The effects of Tf-HE against the myotoxicity caused by venom were evaluated via histological analysis (PND) and creatine kinase (CK) release (BC). Tf-HE was able to reverse the venom paralysis in both preparation types. The contractures to exogenous ACh in BC preparations showed that Tf-HE may act on extrinsic, preserving those intrinsic postsynaptic receptors. There was a positive correlation between CK and morphological changes. The high non-hemolytic saponin content can explain the Tf-HE efficacy against the toxic effects of B. jararacussu venom in vertebrate neuromuscular preparations. |
| Databáze: | OpenAIRE |
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