Improved detection of human papillomavirus infection in genital intraepithelial neoplasia in human immunodeficiency virus positive (HIV +) women by polymerase chain reaction-in situ hybridization
| Autor: | M C Dauge-Geffroy, C. Bedel, Madelenat P, Potet F, F Walker, T Lehy |
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| Rok vydání: | 1996 |
| Předmět: |
Adult
Genital Neoplasms Female In situ hybridization Biology Polymerase Chain Reaction Pathology and Forensic Medicine law.invention Vulva law hemic and lymphatic diseases HIV Seropositivity medicine Humans Serotyping neoplasms Molecular Biology Cervix Papillomaviridae Polymerase chain reaction In Situ Hybridization Intraepithelial neoplasia Papillomavirus Infections virus diseases Cell Biology Virology female genital diseases and pregnancy complications Koilocyte genomic DNA Tumor Virus Infections medicine.anatomical_structure Vagina HIV-1 Female Carcinoma in Situ |
| Zdroj: | Diagnostic molecular pathology : the American journal of surgical pathology, part B. 5(2) |
| ISSN: | 1052-9551 |
| Popis: | The prevalence of genital human papillomavirus (HPV) infection was evaluated in 30 consecutive human immunodeficiency virus (HIV) + women by polymerase chain reaction (PCR)-in situ hybridization (ISH) on paraffin-embedded tissue sections and compared with that found with standard ISH. Biopsies were removed from normal or neoplastic areas in the cervix, vagina, and vulva, and ISH was performed with biotinylated or fluorescein isothiocyanate genomic DNA probes. One probe was used for HPV screening and others for HPV typing (types 6, 11, 16, 18, 31, and 33). Sequences were amplified by the "hot-start" PCR method and followed by standard ISH. Among the 30 HIV + women, 90% scored HPV + in one or several locations by PCR-ISH, whereas only 67% were positive by ISH. Oncogenic HPV types were found in 63% by PCR-ISH and in only 43% by ISH. The same HPV types detected by standard ISH were also recognized by PCR-ISH, but with the latter the signal was amplified. Moreover, some HPV types were found with PCR-ISH but not by ISH. We conclude that PCR-ISH is a valuable and sensitive method for specific detection of HPV. |
| Databáze: | OpenAIRE |
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