Laser Scanning Confocal Microscopy Was Used to Validate the Presence of Burkholderia pseudomallei or B. mallei in Formalin-Fixed Paraffin Embedded Tissues
| Autor: | Jeremy J. Bearss, Susan L. Welkos, Xiankun Zeng, Christopher K. Cote, Carl Soffler, Sylvia R. Trevino, Jennifer L. Dankmeyer, Robert C. Bernhards, Patricia L. Worsham, David M. Waag, Wilson J. Ribot, Kei Amemiya |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Melioidosis 030231 tropical medicine lcsh:Medicine Burkholderia pseudomallei Microbiology 03 medical and health sciences Burkholderia mallei 0302 clinical medicine formalin-fixed paraffin embedded tissue medicine Pathogen General Immunology and Microbiology biology Intracellular parasite lcsh:R Bright-field microscopy Glanders Public Health Environmental and Occupational Health medicine.disease biology.organism_classification Staining 030104 developmental biology Infectious Diseases glanders melioidosis laser scanning confocal microscopy |
| Zdroj: | Tropical Medicine and Infectious Disease, Vol 5, Iss 65, p 65 (2020) |
| ISSN: | 2414-6366 |
| Popis: | Burkholderia pseudomallei and B. mallei are Gram-negative, facultative intracellular bacteria that cause melioidosis and glanders, respectively. Currently, there are no vaccines for these two diseases. Animal models have been developed to evaluate vaccines and therapeutics. Tissues from infected animals, however, must be fixed in formalin and embedded in paraffin (FFPE) before analysis. A brownish staining material in infected tissues that represents the exopolysaccharide of the pathogen was seen by bright field microscopy but not the actual microorganism. Because of these results, FFPE tissue was examined by laser scanning confocal microscopy (LSCM) in an attempt to see the microorganism. Archival FFPE tissues were examined from ten mice, and five nonhuman primates after exposure to B. pseudomallei or B. mallei by LSCM. Additionally, a historical spleen biopsy from a human suspected of exposure to B. mallei was examined. B. pseudomallei was seen in many of the infected tissues from mice. Four out of five nonhuman primates were positive for the pathogen. In the human sample, B. mallei was seen in pyogranulomas in the spleen biopsy. Thus, the presence of the pathogen was validated by LSCM in murine, nonhuman primate, and human FFPE tissues. |
| Databáze: | OpenAIRE |
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