Hyperosmolar treatment of soman-induced brain lesions in mice: evaluation of the effects through diffusion-weighted magnetic resonance imaging and through histology
| Autor: | Christoph Segebarth, Hana Lahrech, Pierre Carpentier, Olivier Montigon, Guy Testylier, Annie Foquin, Frédéric Dorandeu |
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| Přispěvatelé: | Département de toxicologie, Service de Santé des Armées, Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Dojat, Michel |
| Rok vydání: | 2008 |
| Předmět: |
Male
Pathology MESH: Brain Edema medicine.medical_treatment MESH: Brain Diseases Soman MESH: Saline Solution Hypertonic Brain Edema Convulsants Toxicology chemistry.chemical_compound Mice 0302 clinical medicine MESH: Convulsants Edema MESH: Soman MESH: Animals Mannitol MESH: Brain Chemistry Saline 0303 health sciences MESH: Statistics Nonparametric Brain Diseases medicine.diagnostic_test Brain MESH: Neuroprotective Agents 3. Good health Neuroprotective Agents MESH: Mannitol medicine.symptom medicine.medical_specialty Brain damage MESH: Diffusion Magnetic Resonance Imaging Statistics Nonparametric Cerebral edema Lesion 03 medical and health sciences MESH: Brain MESH: Body Water Body Water medicine Animals MESH: Mice 030304 developmental biology Brain Chemistry Saline Solution Hypertonic business.industry Magnetic resonance imaging medicine.disease MESH: Male Hypertonic saline Diffusion Magnetic Resonance Imaging chemistry business 030217 neurology & neurosurgery |
| Zdroj: | Toxicology Toxicology, Elsevier, 2008, 253 (1-3), pp.97-103. ⟨10.1016/j.tox.2008.08.016⟩ |
| ISSN: | 0300-483X |
| DOI: | 10.1016/j.tox.2008.08.016⟩ |
| Popis: | International audience; PURPOSE: A convulsive dose of soman induces seizure-related brain damage (SRBD), including cerebral edema (CE) and neuronal loss. In the present study on soman-intoxicated mice, we applied diffusion-weighted magnetic resonance imaging (DW-MRI) and quantitative histology, and we measured brain water content to investigate the antiedematous and neuroprotective efficacies of two hyperosmolar treatments: mannitol (Mann) and hypertonic saline (HTS). METHODS: Mice intoxicated with soman (172 microg/kg after a protective pretreatment) were administered 1 min and 5-h post-challenge an i.v. bolus of saline, of Mann or of HTS. 1 day later, mice were examined with DW-MRI and then sacrificed for brain histology. Additional animals were intoxicated and treated similarly for the measurement of the brain water content (dry/wet weight method). RESULTS: In intoxicated controls, a significant decrease of the apparent diffusion coefficient (ADC), numerous damaged (eosinophilic) cells, high edema scores, and a significant increase in brain water content were detected 24-h post-challenge in sensitive brain structures. These soman-induced changes were not significantly modified by treatment with Mann or HTS. CONCLUSIONS: Treatment with hyperosmolar solutions did not reduce the effects of soman on ADC, on cell damage and on CE. Therefore, despite similar treatment protocols, the prominent protection by Mann that was previously demonstrated by others in poisoned rats, was not reproduced in our murine model. |
| Databáze: | OpenAIRE |
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