Cell wall canals formed upon growth of Candida maltosa in the presence of hexadecane are associated with polyphosphates
| Autor: | Tatiana G. Rusakova, Tatiana Kulakovskaya, Vladimir V. Dmitriev, Anton Zvonarev, David E. Crowley, Lydia P Lichko, L. P. Ryazanova |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Indoles 3 3'-Diaminobenzidine Diamines Biology Hexadecane Applied Microbiology and Biotechnology Microbiology Cell wall 03 medical and health sciences chemistry.chemical_compound Microscopy Electron Transmission Cell Wall Polyphosphates Alkanes Oxidative enzyme Candida chemistry.chemical_classification Staining and Labeling 030102 biochemistry & molecular biology Polyphosphate General Medicine Phosphate Yeast Acid Anhydride Hydrolases Culture Media Staining Glucose 030104 developmental biology Enzyme chemistry Biochemistry |
| Zdroj: | FEMS Yeast Research. 17 |
| ISSN: | 1567-1364 |
| Popis: | Canals are supramolecular complexes observed in the cell wall of Candida maltosa grown in the presence of hexadecane as a sole carbon source. Such structures were not observed in glucose-grown cells. Microscopic observations of cells stained with diaminobenzidine revealed the presence of oxidative enzymes in the canals. 4΄,6΄-diamino-2-phenylindole staining revealed that a substantial part of cellular polyphosphate was present in the cell wall of cells grown on hexadecane in condition of phosphate limitation. The content and chain length of polyphosphates were higher in hexadecane-grown cells than in glucose grown ones. The treatment of cells with yeast polyphosphatase PPX1 resulted in the decrease of the canal size. These data clearly indicated that polyphosphates are constituents of canals; they might play an important role in the canal structure and functioning. |
| Databáze: | OpenAIRE |
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