Correlation between cardiac oxidative stress and myocardial pathology due to acute and chronic norepinephrine administration in rats
Autor: | Daniela Cerretani, Angela Bruna Maffione, Carmela Fiore, Cristoforo Pomara, Pasini Franco Laghi, Cristina Nencini, Lucia Micheli, Stefano D'Errico, Anna Ida Fiaschi, Emanuela Turillazzi, Giorgio Giorgi, Pietro Euea Lazzerini, Margherita Neri, Irene Riezzo, Giancarlo Bruni, Vittorio Fineschi |
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Přispěvatelé: | Neri, M, Cerretani, D, Fiaschi, Ai, Laghi, Pf, Lazzaroni, Pe, Mafiose, Ab, Micheli, L, Bruni, G, Nencini, C, Giorgi, G, D'Errico, S, Fiore, C, Pomara, C, Riezzo, I, Turillazzi, E, Fineschi, V |
Jazyk: | angličtina |
Rok vydání: | 2007 |
Předmět: |
Male
Glutathione reductase Apoptosis medicine.disease_cause chemistry.chemical_compound Electrocardiography cardiac antioxidant cellular defence system Malondialdehyde chemistry.chemical_classification Glutathione Disulfide Glutathione peroxidase Articles Immunohistochemistry Glutathione Reductase Molecular Medicine Cardiac antioxidant cellular defence system oxidative stress myocardial pathology norepinephrine Adrenergic alpha-Agonists medicine.medical_specialty β1-adrenergic receptors Cytokines Norepinephrine Animals Drug Administration Schedule Glutathione Peroxidase Lipid Peroxidation Myocardium Oxidative Stress Rats Rats Wistar Reactive Oxygen Species Receptor Cross-Talk Receptors Adrenergic beta-1 Superoxide Dismutase Biochemistry Genetics and Molecular Biology (all) Biochemistry β Socio-culturale Biology Superoxide dismutase Internal medicine medicine adrenergic receptors Reactive oxygen species 1 Cell Biology Glutathione norepinephrine cardiac antioxidant cellular defence system β1-adrenergic receptors cytokines cytokines Endocrinology chemistry biology.protein Glutathione disulfide Oxidative stress |
Zdroj: | Journal of Cellular and Molecular Medicine |
ISSN: | 1582-4934 1582-1838 |
Popis: | Background: To investigate the cardiotoxic role of reactive oxygen species (ROS) and of products derived from catecholamines auto-oxidation, we studied: (1) the response of antioxidant cardiac cellular defence systems to oxidative stress induced by norepinephrine (NE) administration, (2) the effect of NE administration on cardiac β1-adrenergic receptors by means of receptor binding assay, (3) the cellular morphological alterations related to the biologically cross-talk between the NE administration and cytokines [tumor necrosis factor-alpha (TNF-α), monocyte chemotactic protein-1 (MCP-1), interleukins IL6, IL8, IL10]Methods and Results: A total of 195 male rats was used in the experiment. All animals underwent electrocardiogram (EKG) before being sacrificed. The results obtained show that NE administration influences the antioxidant cellular defence system significantly increasing glutathione peroxidase (GPx) activity, glutathione reductase (GR) and superoxide dismutase (SOD). The oxidized glutathione (GSH/GSSG) ratio significantly decreases and malondialdehyde (MDA) levels increase showing a state of lipoperoxidation of cardiac tissue. We describe a significant apoptotic process randomly sparse in the damaged myocardium and the effect of ROS on the NE-mediated TNF-α, MCP-1, and IL6, IL8, IL10 production. Conclusions: Our results support the hypothesis that catecholamines may induce oxidative damage through reactive intermediates resulting from their auto-oxidation, irrespective of their interaction with adrenergic receptors, thus representing an important factor in the pathogenesis of catecholamines-induced cardiotoxicity. The rise of the cardioinhibitory cytokines may be interpreted as the adaptive response of jeopardized myocardium with respect to the cardiac dysfunction resulting from NE injection. |
Databáze: | OpenAIRE |
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