Optical Imaging-Based Guidance of Viral Microinjections and Insertion of a Laminar Electrophysiology Probe Into a Predetermined Barrel in Mouse Area S1BF
| Autor: | Victor M. Mocanu, Amir Shmuel |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Materials science Microinjections barrel field Cognitive Neuroscience Neuroscience (miscellaneous) Neurosciences. Biological psychiatry. Neuropsychiatry Optogenetics Neural Circuits functional localization Photostimulation 03 medical and health sciences Cellular and Molecular Neuroscience Mice 0302 clinical medicine Cortex (anatomy) medicine Methods Premovement neuronal activity Animals laminar probe 030304 developmental biology Cerebral Cortex Neurons 0303 health sciences Brain Mapping Optical Imaging viral microinjection Somatosensory Cortex Neurophysiology Sensory Systems Electrophysiological Phenomena Electrophysiology Mice Inbred C57BL medicine.anatomical_structure Cerebral cortex Neurovascular Coupling Female optical imaging of intrinsic signals neurophysiology Neuroscience 030217 neurology & neurosurgery RC321-571 cortical columns |
| Zdroj: | Frontiers in Neural Circuits Frontiers in Neural Circuits, Vol 15 (2021) |
| ISSN: | 1662-5110 |
| Popis: | Wide-field Optical Imaging of Intrinsic Signals (OI-IS; Grinvald et al., 1986) is a method for imaging functional brain hemodynamic responses, mainly used to image activity from the surface of the cerebral cortex. It localizes small functional modules – such as cortical columns – with great spatial resolution and spatial specificity relative to the site of increases in neuronal activity. OI-IS is capable of imaging responses either through an intact or thinned skull or following a craniotomy. Therefore, it is minimally invasive, which makes it ideal for survival experiments. Here we describe OI-IS-based methods for guiding microinjections of optogenetics viral vectors in proximity to small functional modules (S1 barrels) of the cerebral cortex and for guiding the insertion of electrodes for electrophysiological recording into such modules. We validate our proposed methods by tissue processing of the cerebral barrel field area, revealing the track of the electrode in a predetermined barrel. In addition, we demonstrate the use of optical imaging to visualize the spatial extent of the optogenetics photostimulation, making it possible to estimate one of the two variables that conjointly determine which region of the brain is stimulated. Lastly, we demonstrate the use of OI-IS at high-magnification for imaging the upper recording contacts of a laminar probe, making it possible to estimate the insertion depth of all contacts relative to the surface of the cortex. These methods support the precise positioning of microinjections and recording electrodes, thus overcoming the variability in the spatial position of fine-scale functional modules. |
| Databáze: | OpenAIRE |
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