Covalently-bound heparin makes collagen thromboresistant
| Autor: | Anita Driessen, Theo Lindhout, Simone J.H. Wielders, Michel Verhoeven, Marc Hendriks, Jeffrey F.W. Keuren |
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| Přispěvatelé: | Biochemie, Pathologie, Literature & Art, RS: FASoS WTMC |
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Surface Properties
Thrombogenicity Antithrombins Collagen Type I Thrombin Platelet Adhesiveness Coated Materials Biocompatible Platelet adhesiveness Protein Interaction Mapping medicine Animals Humans Platelet Platelet activation Annexin A5 Blood Coagulation Chemistry Heparin Antithrombin Periodic Acid Thrombosis Oxidants Platelet Activation Blood Coagulation Factors Immunity Innate Cross-Linking Reagents Immunology Hemorheology Biophysics Cattle Cardiology and Cardiovascular Medicine Oxidation-Reduction medicine.drug Protein Binding |
| Zdroj: | Arteriosclerosis Thrombosis and Vascular Biology, 24(3), 613-617. LIPPINCOTT WILLIAMS & WILKINS |
| ISSN: | 1079-5642 |
| DOI: | 10.1161/01.atv.0000116026.18945.66 |
| Popis: | Objective— Blood compatibility of artificial surfaces depends on their immunogenic and thrombogenic properties. Collagen’s weak antigenicity makes it an attractive candidate for stent coatings or fabrication of vascular grafts. However, the thrombogenic nature of collagen limits its application. We examined whether heparinization can make collagen more thromboresistant. Methods and Results— Collagen was heparinized by crosslinking collagen with extensively periodate oxidized heparin and/or by covalently bonding of mildly periodate oxidized heparin. Both ways of heparinization have no effect on platelet adhesion and could not abolish induction of platelet procoagulant activity. However, thrombin generation was completely prevented under static and flow conditions. The functionality of immobilized heparin was confirmed by specific uptake of antithrombin, 13.5±4.7 pmol/cm 2 and 1.95±0.21 pmol/cm 2 for mildly and heavily periodated heparin, respectively. Conclusions— These results indicate that immobilization of heparin on collagen, even as a crosslinker, is a very effective way to prevent surface thrombus formation. These data encourage the application of heparinized collagen as stent-graft material in animal and eventually human studies. |
| Databáze: | OpenAIRE |
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