Prevalence of Myeloperoxidase Gene Expression in Infant Acute Lymphocytic Leukemia
| Autor: | Eric D. Austin, Michael J. Borowitz, Allen R. Chauvenet, Nasrollah Hakami, Carlos S. Alvarado, Wei Guo Zhao, Andrew J. Carroll, Garth E. Austin |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Myeloid animal diseases Gene Expression Biology Immunophenotyping Precursor B-Cell Lymphoblastic Leukemia-Lymphoma hemic and lymphatic diseases Precursor cell Acute lymphocytic leukemia Gene expression medicine Humans Peroxidase Lymphoblast Infant RNA General Medicine Precursor Cell Lymphoblastic Leukemia-Lymphoma Blotting Northern medicine.disease Molecular biology medicine.anatomical_structure Myeloperoxidase Immunology biology.protein Female |
| Zdroj: | American Journal of Clinical Pathology. 110:575-581 |
| ISSN: | 1943-7722 0002-9173 |
| Popis: | The enzyme myeloperoxidase (MPO; donor: H2O2 oxidoreductase, EC1.11.1.7) is a well-established marker of myeloid differentiation. Most myeloid leukemias express MPO enzyme activity at the light microscopic level, whereas lymphoid leukemias characteristically lack such expression. However, the diagnostic significance of MPO RNA expression or of immunohistochemically detectable MPO protein expression in leukemic blasts is unclear. We studied the prevalence and diagnostic significance of MPO RNA and protein expression in 57 cases of MPO enzyme-negative infant B-precursor acute lymphocytic leukemia (ALL), since the blast cells in this condition have been reported to show a high incidence of coexpression of myeloid-associated antigens. MPO expression was compared with other clinical and laboratory parameters. Of the cases examined, 56% showed detectable MPO expression at the RNA or protein level or both. Most positive cases showed MPO protein in many leukemic blasts, whereas a few cases showed substantial MPO protein expression in only a few blast cells. MPO expression showed no significant correlation with other markers of myeloid differentiation. Leukemic lymphoblasts in infant ALL frequently express MPO at the RNA or protein level; this expression does not imply an overall myeloid phenotype. The leukemic blasts in infant ALL may derive from an immature hematopoietic precursor cell not fully committed to lymphoid differentiation. |
| Databáze: | OpenAIRE |
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