Enrichment and physiological characterization of an anaerobic ammonium-oxidizing bacterium ‘Candidatus Brocadia sapporoensis’
Autor: | Yuko Narita, Satoshi Okabe, Lei Zhang, Zen-ichiro Kimura, Muhammad Ali, Takao Fujii |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Brocadia fulgida ved/biology.organism_classification_rank.species Hydroxylamine 010501 environmental sciences 01 natural sciences Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences chemistry.chemical_compound Bioreactors Microscopy Electron Transmission RNA Ribosomal 16S Ammonium Compounds Ammonium Nitrite Ecology Evolution Behavior and Systematics 0105 earth and related environmental sciences biology ved/biology Vitamin K 2 biology.organism_classification 16S ribosomal RNA Anoxic waters Planctomycetales Hydrazines 030104 developmental biology chemistry Anammox Oxidation-Reduction Genome Bacterial Bacteria |
Zdroj: | Systematic and Applied Microbiology. 40:448-457 |
ISSN: | 0723-2020 |
Popis: | We successfully enriched a novel anaerobic ammonium-oxidizing (anammox) bacterium affiliated with the genus 'Candidatus Brocadia' with high purity (>90%) in a membrane bioreactor (MBR). The enriched bacterium was distantly related to the hitherto characterized 'Ca. Brocadia fulgida' and 'Ca. Brocadia sinica' with 96% and 93% of 16S ribosomal RNA gene sequence identity, respectively. The bacterium exhibited the common structural features of anammox bacteria and produced hydrazine in the presence of hydroxylamine under anoxic conditions. The temperature range of anammox activity was 20-45°C with a maximum activity at 37°C. The maximum specific growth rate (μmax) was 0.0082h-1 at 37°C, corresponding to a doubling time of 3.5 days. The half-saturation constant (KS) for nitrite was 5±2.5μM. The anammox activity was inhibited by nitrite (IC50=11.6mM) but not by formate and acetate. The major respiratory quinone was identified to be menaquinone-7 (MK-7). The enriched anammox bacterium shared nearly half of genes with 'Ca. Brocadia sinica' and 'Ca. Brocadia fulgida'. The enriched bacterium showed all known physiological characteristics of anammox bacteria and can be distinguished from the close relatives by its 16S rRNA gene sequence. Therefore, we proposed the name 'Ca. Brocadia sapporoensis' sp. nov. |
Databáze: | OpenAIRE |
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