In vivo and in vitro evidence for an association between the route-specific transmission of HHV-8 and the virus genotype
| Autor: | A. Scaccino, Alberto Faggioni, Antonio Angeloni, Francesco Broccolo, Luca Ceccherini-Nelli, Francesca Cottoni, B Matteoli |
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| Přispěvatelé: | Matteoli, B, Broccolo, F, Scaccino, A, Cottoni, F, Angeloni, A, Faggioni, A, Ceccherini Nelli, L |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
Saliva Genotype viruses Molecular Sequence Data Biology Peripheral blood mononuclear cell classic ks Virus Cell Line hhv-8 genotype HEK293 Cell Virology Humans Seroprevalence Amino Acid Sequence Sarcoma Kaposi Phylogeny hhv-8 Epithelial Cell Herpesviridae Infection cellular tropism transmission virus diseases Epithelial Cells Herpesviridae Infections Sequence Analysis DNA Viral Load Viral Tropism HEK293 Cells Infectious Diseases Italy Cell culture DNA Viral Herpesvirus 8 Human Immunology Leukocytes Mononuclear Tissue tropism Female Viral load Human |
| Popis: | The study was performed to determine if there is an association between the genotype and transmission of HHV-8 types A and C. These HHV-8 subtypes are prevalent in the area of North of Sardinia, which is an island off west Italy's mainland that has a high HHV-8 seroprevalence (35%). Blood and saliva samples from 30 patients with classic Kaposi's sarcoma who were lifetime residents of North Sardinia were analyzed to identify the HHV-8 genotype and quantitate the viral load. Genotype A, especially A1 subtype, was found more frequently (9/30 patients) and had a significantly higher viral load in saliva compared to blood (P = 0.029), where type C was found more frequently but with a viral load lower than 103 copies/ml. To determine if there is a correlation between the viral genotype and cellular tropism, type A1 and C3 HHV-8 viral particles were obtained from cell lines BCBL1 and BC3 infected chronically with HHV-8 A1 and C3 genotypes respectively and used to infect HEK293 epithelial origin cells and PBMCs in vitro. The data indicate that the A1 HHV-8 genotype is tropic and replicates at higher levels in the epithelial cell lines. J. Med. Virol. 84:786–791, 2012. © 2012 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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