Acute hypoxia upregulates NOS gene expression in rats
| Autor: | Karin Schricker, Armin Kurtz, Michael Pfeifer, Bernhard Gess |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
medicine.medical_specialty Transcription Genetic Physiology Biology Kidney Gene Expression Regulation Enzymologic Nitric oxide Rats Sprague-Dawley chemistry.chemical_compound Physiology (medical) Internal medicine Gene expression medicine Animals RNA Messenger Respiratory system Hypoxia Erythropoietin Lung Regulation of gene expression Carbon Monoxide Myocardium Hypoxia (medical) Actins Rats Oxygen Endocrinology medicine.anatomical_structure Liver chemistry Organ Specificity Circulatory system Nitric Oxide Synthase medicine.symptom |
| Zdroj: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 273:R905-R910 |
| ISSN: | 1522-1490 0363-6119 |
| DOI: | 10.1152/ajpregu.1997.273.3.r905 |
| Popis: | This study aimed to investigate the influence of acute tissue hypoxygenation on the expression of NO synthase (NOS) genes in vivo. To this end, male Sprague-Dawley rats were exposed either to 9% oxygen or to 0.1% carbon monoxide for 6 h, and mRNA levels of NOS-I, -II, and -III in kidneys, livers, lungs, and left and right heart ventricles were assayed by ribonuclease protection. For comparison, mRNA levels of erythropoietin were also measured in these tissues. NOS-III mRNA was highly abundant in all organs investigated. NOS-II mRNA was detected in lungs and hearts but not in kidneys and livers. NOS-I mRNA was found in kidneys, lungs, and hearts but not in livers. NOS-III mRNA levels were upregulated by hypoxia in all tissues examined, with the least effect (1.2-fold) in the left ventricle and the greatest effect (2.6-fold) in the lung. NOS-II mRNA was substantially downregulated in the ventricles by both treatments but not changed in the lung. NOS-I mRNA was upregulated by carbon monoxide in kidneys and lungs and by 9% oxygen in the lung. These findings suggest that NOS-III and possibly also NOS-I gene expression behave like oxygen-regulated genes, whereas the general effect of tissue hypoxygenation on NOS-II gene expression is less clear. Because NOS-III is primarily expressed in endothelial cells, a general upregulation of NOS in these cells may be of relevance for the regulation and maintenance of blood flow through hypoxic tissues. |
| Databáze: | OpenAIRE |
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