ISFET-based Aptasensor for Thrombin Detection Using Horseradish Peroxidase
| Autor: | Natalia Komarova, Mariia Andrianova, Alexander Kuznetsov, Vitaliy Grudtsov, Evgeniy V. Kuznetsov |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
chemistry.chemical_classification
Chromatography biology Aptamer Biomolecule 010401 analytical chemistry Gate dielectric Analytical chemistry 02 engineering and technology General Medicine 021001 nanoscience & nanotechnology 01 natural sciences Horseradish peroxidase 0104 chemical sciences Thrombin chemistry medicine biology.protein ISFET 0210 nano-technology Biosensor Engineering(all) medicine.drug |
| Zdroj: | Procedia Engineering. 174:1084-1092 |
| ISSN: | 1877-7058 |
| DOI: | 10.1016/j.proeng.2017.01.261 |
| Popis: | Use of an ion-sensitive field-effect transistor (ISFET) as a signal transducer in a chemical analytical system is of great scientific and application interest. Modification of the gate dielectric surface with biomolecules makes ISFET a universal platform for different applications. In this paper horseradish peroxidase (HRP, EC 1.11.1.7) was used as a label for determination of interaction between thrombin and its aptamer on the ISFET surface. The approach for thrombin detection was based on the replacement of complementary sDNA-probe containing HRP from immobilized aptamer (sDNA) with thrombin, and monitoring residual HRP activity. The biosensor was shown to detect thrombin at the level of 7·10 -7 M. |
| Databáze: | OpenAIRE |
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