Factors effecting expression of vaccines in microalgae
| Autor: | Katie Greenham, Raymond Surzycki, Flora Dibal, Tarek Ajam, Kaoru Kitayama, Richard E. Wagner, Stefan J. Surzycki, Jean-David Rochaix |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Genotype
medicine.medical_treatment Blotting Western Bioengineering Biology Applied Microbiology and Biotechnology Animals Genetically Modified medicine Animals Plastid Codon Pharmacology Genetics Vaccines Synthetic Protease General Immunology and Microbiology business.industry Chlamydomonas food and beverages General Medicine medicine.disease biology.organism_classification Biotechnology Genetically modified organism Chloroplast Transformation (genetics) Protein toxicity business |
| Zdroj: | Biologicals. 37:133-138 |
| ISSN: | 1045-1056 |
| DOI: | 10.1016/j.biologicals.2009.02.005 |
| Popis: | PhycoBiologics is developing an oral vaccine delivery system using vaccines expressed in the chloroplast of microalgae. Despite many advances in plastid transformation technology, levels of expression remain inconsistent. We have concluded that the main factors affecting the level of recombinant protein expression in the chloroplast of Chlamydomonas are: codon optimization, protease activity, protein toxicity and transformation-associated genotypic modification. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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