Implications of the RhoA/Rho associated kinase pathway and leptin in primary uterine inertia in the dog
| Autor: | Iris M Reichler, Sandra Goericke-Pesch, Aykut Gram, Orsolya Balogh, Mariusz P. Kowalewski, Stefanie Keller, Bianca Lourdes Frehner |
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| Přispěvatelé: | University of Zurich |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Leptin
medicine.medical_specialty RHOA 10077 Institute of Veterinary Anatomy Uterus In situ hybridization Biology Contractility Canine 03 medical and health sciences Dogs 0302 clinical medicine Pregnancy Internal medicine medicine Animals ROCK1 ROCK2 030304 developmental biology rho-Associated Kinases 0303 health sciences 030219 obstetrics & reproductive medicine 630 Agriculture Parturition Myometrium Dystocia 10187 Department of Farm Animals Endocrinology medicine.anatomical_structure biology.protein Receptors Leptin 570 Life sciences biology Original Article Female Animal Science and Zoology 1103 Animal Science and Zoology Uterine Inertia rhoA GTP-Binding Protein Signal Transduction |
| Zdroj: | Frehner, B L, Reichler, I M, Kowalewski, M P, Gram, A, Keller, S, Goericke-Pesch, S & Balogh, O 2021, ' Implications of the RhoA/Rho associated kinase pathway and leptin in primary uterine inertia in the dog ', The Journal of reproduction and development, vol. 67, no. 3, pp. 207-215 . https://doi.org/10.1262/jrd.2020-141 The Journal of Reproduction and Development |
| DOI: | 10.1262/jrd.2020-141 |
| Popis: | The underlying functional and molecular changes in canine primary uterine inertia (PUI) are still not clarified. Leptin (Lep) and obesity negatively affect uterine contractility in women, partly mediated by the RhoA/Rho associated kinase pathway, affecting myometrial calcium sensitization. We hypothesized that increased uterine Lep/Lep receptor (LepR) or decreased RhoA/Rho associated kinase expression contributes to PUI in dogs, independent of obesity. Dogs presented for dystocia were grouped into PUI (n = 11) or obstructive dystocia (OD, still showing strong labor contractions; n = 7). Interplacental full-thickness uterine biopsies were collected during Cesarean section for relative gene expression (RGE) of RhoA, its effector kinases (ROCK1, ROCK2), Lep and LepR by qPCR. Protein and/or mRNA expression and localization was evaluated by immunohistochemistry and in situ hybridization. RGE was compared between groups by one-way ANOVA using body weight as covariate with statistical significance at P < 0.05. Uterine ROCK1 and ROCK2 gene expression was significantly higher in PUI than OD, while RhoA and Lep did not differ. LepR RGE was below the detection limit in five PUI and all OD dogs. Litter size had no influence. Lep, LepR, RhoA, ROCK1, ROCK2 protein and/or mRNA were localized in the myometrium and endometrium. Uterine protein expression appeared similar between groups. LepR mRNA signals appeared stronger in PUI than OD. In conclusion, lasting, strong labor contractions in OD likely resulted in downregulation of uterine ROCK1 and ROCK2, contrasting the higher expression in PUI dogs with insufficient contractions. The Lep-LepR system may affect uterine contractility in non-obese PUI dogs in a paracrine-autocrine manner. |
| Databáze: | OpenAIRE |
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